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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 朴涌基
- 발행연도
- 2014
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
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이 논문의 연구 히스토리 (5)
2014
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In this study, we investigated the anti-asthmatic effects of Agastachis Herba water (AH-W) extract on compound 48/80-induced degranulation and histamine release in human mast cell, HMC-1 and ovalbumin (OVA)-induced asthmatic mice.
HMC-1 cells were treated with the different concentrations of AH-W extract in the presence or absence of compound 48/80 for 30 min, and then measured their degranulation and histamine release in culture supernatants by ELISA.
Mice were sensitized (0, 7, and 14 days) with OVA and airway challenged (21, 23, 25, 27, and 29 days) for induction of asthmatic responses. AH-W extract at doses of 100 and 300 mg/kg/body weight (bw) was orally administered during OVA challenge once per a day. The levels of immunoglobulin (Ig) E, and Th1/Th2 cytokines (IFN-γ and IL-4) were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed by hematoxylin and eosin (H&E) and Periodic Acid Schiff(PAS) staining.
The treatment of AH-W extract significantly decreased the histamine levels through inhibiting compound 48/80-induced degranulation in HMC-1 cells. In addition, The administration of AH-W extract at does of 100 and 300 mg/kg bw significantly decreased the serum levels of OVA-specific IgE compared with those of OVA control group. In H&E and PAS staining, AH-W extract inhibited OVA-induced airway inflammation and inflammatory cells infiltration, and also protected lung tissues against histopathological damages such as bronchiole epithelial desquamation, goblet cell hyperplasia, mucin releasing.
These results indicate that AH-W extract may improve asthmatic symptoms through mast cell stabilization and inhibiting the lung inflammation in bronchial asthmatic.
HMC-1 cells were treated with the different concentrations of AH-W extract in the presence or absence of compound 48/80 for 30 min, and then measured their degranulation and histamine release in culture supernatants by ELISA.
Mice were sensitized (0, 7, and 14 days) with OVA and airway challenged (21, 23, 25, 27, and 29 days) for induction of asthmatic responses. AH-W extract at doses of 100 and 300 mg/kg/body weight (bw) was orally administered during OVA challenge once per a day. The levels of immunoglobulin (Ig) E, and Th1/Th2 cytokines (IFN-γ and IL-4) were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed by hematoxylin and eosin (H&E) and Periodic Acid Schiff(PAS) staining.
The treatment of AH-W extract significantly decreased the histamine levels through inhibiting compound 48/80-induced degranulation in HMC-1 cells. In addition, The administration of AH-W extract at does of 100 and 300 mg/kg bw significantly decreased the serum levels of OVA-specific IgE compared with those of OVA control group. In H&E and PAS staining, AH-W extract inhibited OVA-induced airway inflammation and inflammatory cells infiltration, and also protected lung tissues against histopathological damages such as bronchiole epithelial desquamation, goblet cell hyperplasia, mucin releasing.
These results indicate that AH-W extract may improve asthmatic symptoms through mast cell stabilization and inhibiting the lung inflammation in bronchial asthmatic.
목차
Ⅰ. 서 론 = 1Ⅱ. 재료 및 방법 = 51. 재료 = 5(1) 약재 = 5(2) 실험동물 = 5(3) 세포주 = 5(4) 시약 및 기기 = 52. 방법 = 6(1) 약물 제조 = 6(2) 비만세포 배양 = 6(3) 비만세포에서의 독성평가 = 7(4) 비만세포에서의 탈과립 평가 = 7(5) 비만세포에서의 히스타민 농도 측정 = 8(6) 난알부민-유도 천식 마우스 모델 제작 = 8(7) 천식 마우스의 혈청 내 난알부민-특이 IgE 농도 측정 = 10(8) 천식 마우스의 혈청 내 사이토카인 농도 측정 = 10(9) 천식 마우스 폐 조직의 병리표본 제작, 염색 및 관찰 = 11(10) 통계학적 검정 = 12III. 결과 = 131. 비만세포에서의 효능평가 = 131) 세포독성 = 132) 비만세포의 탈과립 억제 효과 = 153) 비만세포로부터 히스타민 분비 억제 효과 = 172. 난알부민-유도 천식 마우스에서의 효능평가 = 191) 혈청 내 난알부민 특이-IgE 생성 억제 효과 = 192) 혈청 내 IL-4 분비 억제 효과 = 213) 혈청 내 IFN-γ 분비 억제 효과 = 234) 폐 조직에서의 염증 억제 효과 = 255) 폐 조직에서의 점액분비 억제효과 = 27Ⅳ. 고 찰 = 29Ⅴ. 결 론 = 37참 고 문 헌 = 38Abstract = 42
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