지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 한증술.
- 발행연도
- 2014
- 저작권
- 경북대학교 논문은 저작권에 의해 보호받습니다.
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이 논문의 연구 히스토리 (3)
2014
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- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
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초록· 키워드
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Chrysanthemum is one of the most important commercial cut flowers in the world. In summer, heat stress by high temperature has effects on chrysanthemum like low productivity, flowering delay and discoloration. Monothiol glutaredoxins play important roles in maintaining redox homeostasis in living cells and share some conserved function across species. Arabidopsis thaliana monothiol glutaredoxin AtGRX3 is critical for protection from oxidative stress in cytosol and/or nucleus. To improve the tolerance to heat stress in chrysanthemum, independent twelve lines constitutively over-expressing AtGRX3 that isolated from A. thaliana were generated via Agrobacterium-mediated genetic transformation technology. All lines were characterized by polymerase chain reaction, Southern hybridization, semi-quantitative reverse transcription-PCR, and bioassays. Over-expression of AtGRX3 in Chrysanthemum plants enhanced photosynthetic performance and insistence of cell membrane permeability, and decreased oxidative damage in addition to promotion of plant growth under heat stresses. These findings suggest a specific protective role of the redox protein against high temperature stress, and provide a genetic engineering strategy to improve crop thermotolerance.
목차
I. Introduction 1II. Materials and Methods 6Plant material 6Plant regeneration procedure 7Kanamycin-resistance assay 8Bacterial strain and plasmid construction for genetic transformation 8Agrobacterium-mediated genetic transformation procedure 10DNA extraction and polymerase chain reaction (PCR) analysis 12Ploidy analysis of regenerated plant 13Southern hybridization analysis 13Semi-quantitative reverse transcription-PCR (RT-PCR) 15High temperature treatment for heat stress 16Chlorophyll fluorescence quenching analysis 16Electrolyte leakage measurement 17Histochemical detection of H2O2 using 3,3’-diaminobenzidine (DAB) 18Statistical analysis 19III. Results and Discussion 20Selection of cultivars and phytohormone combination for shoot regeneration 20Responses of cultures to antibiotics, kanamycin 23Generation of transgenic plant and molecular analysis 26Phenotype observation of AtGRX3-expressing plant 33Response of AtGRX3-expressing plant to heat stress 35H2O2 accumulation of AtGRX3-expressing plant encountering heat stress 41IV. References 46V. 국문초록 54
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