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논문 기본 정보

자료유형
학위논문
저자정보

Jana Jeevan Rameneni (충남대학교, 충남대학교 대학원)

지도교수
임용표(Yong Pyo Lim)
발행연도
2014
저작권
충남대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (3)

초록· 키워드

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The involvement of MADS-box transcription factors in the development of seeds, flowers, and fruit is well known. Large number of MADS-box genes have been characterized and reported in major plants such as Arabidopsis thaliana (107), Oryza sativa (75), Zea mays (75), and Cucumis sativus (43). However, there is little information about MADS-box genes in the economically and morphologically important genus Brassica. Thus, we performed a series of computational analyses on the recently published Brassica rapa genome and identified 167 potential MADS-box genes. Chromosomal localization of these genes revealed many duplicate genes and 10 tandem repeats from B. rapa chromosomes, though none on A04, A08, and A10.Neighbor-joining phylogenetic analysis was performed between B. rapa, A. thaliana, O. sativa, Z. mays, and C. sativus genes, and type-I and type-II groups were differentiated by their gene clades. Based on their phylogeny and functional characters, type-I group was divided into three clades and type-II group into 14clades. Prediction and comparison of intron and exon patterns of MADS-box genes authenticates the major difference between the groups. Semi-quantitative RT-PCR analysis was carried out for 25 functionally categorized BrMADS-box genes and comparatively studied among buds, sepals, petals, stamens, carpels, and siliques of the parental line Rapid Cycling Brassica rapa (RcBr). The structural and functional annotation, as well as expressional profiling of BrMADS in floral organs in this study will be the basis of further functional validation and molecular breeding of B. rapa crops.

목차

I. Introduction 1
II. Materials and Methods 6
1. Identification of MADS-box genes in B. rapa 6
2. Intron/exon structure 6
3. Identification of conserved motifs 6
4. Annotation of gene ontology 7
5. Phylogenetic analysis 7
6. Expression analysis of MADS box genes in B. rapa 7
III. Results and Discussion 10
1. Identification and chromosomal localization of MADS-box genes in the genome of
B. rapa 10
2. Intron/Exon structure and duplication analysis 19
3. Comparative phylogenetic analysis and conserved motif identification of MADS- box genes in B. rapa, A. thaliana, O. sativa, Z. mays and C. sativus 22
4. Functional classification of B. rapa MADS-box genes 30
5. Expression analysis of 25 B. rapa MADS-box genes 33
IV. Conclusion 36
V. Literature cited 37
Abstract 42
Korean Abstract 44
Acknowledgement 45

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