For the development of feasible retinal prosthesis, one important element is acquiring proper judging tool if electrical stimulus leads to patient’s perception. If there is VEP response to electrical stimulus in animal, it means that the stimulus effectively evokes response in visual cortex. In-vivo VEP recording on V1 cortex provides better information about animal’s perception than in-vitro retinal recording we have been performing in our lab. Therefore, in this study, we established VEP recording on V1 cortex using BioPAC system. After anesthesia, wild-type (C57BL/6J strain) mice (n=7) were secured to stereotaxic apparatus (Harvard Apparatus, USA). For the recording of VEP, the stainless steel needle electrode (impedance: 2-5 kΩ) was positioned on the surface of the cortex through the burr hole at 2.5 mm lateral and 4.6 mm caudal to bregma. DA 100C and EEG 100C BioPAC modules were used for the trigger signal recording and VEP recording, respectively. When left eye was blocked by black cover and right eye was stimulated by flash light using HMsERG (RetVet Corp, USA), VEP response at left V1 cortex was detected, but no response at right V1 cortex. Amplitude and latency of P1 peak of VEP recording varied according to the depth of the electrode on V1 cortex. From the surface upto 600 μm depth, P1-N1 peak amplitude increased (Gain: 20,000, 173.62 ± 17.61 μV, n=5), while deeper than 600 μm, P1-N1 peak amplitude decreased. The deeper the insertion depth of the electrode, the latency of N1 peaks tends to be delayed. However, there was no statistically significant difference among the latencies of N1 peaks (P?0.05, ANOVA). Our VEP recording data might be used as a guideline for electrically evoked potential (EEP) recording experiment in near future.