지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 장준복
- 발행연도
- 2015
- 저작권
- 중부대학교 논문은 저작권에 의해 보호받습니다.
이용수1
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
This study was performed to investigate the physiological activity, α-glucosidase inhibitory and structural analysis of extracts from Maackia amurensis bark.
Bark extracts of Maackia amurensis were prepared using 70% methanol. Methanol extracts were fractionated to hexane, chloroform, ethyl acetate, butyl alcohol, water layer and investigated the physiological activity, α-glucosidase inhibitory and structural analysis.
The polyphenol contents of 70% methanol extracts from Maackia amurensis bark was higher by 14.44±0.44 mg/g, IC50 values of electron donating ability and α-glucosidase inhibitory were lower in each of 194.15±2.96 ㎍/mL and 322.19±2.63 ㎍/mL.
The total polyphenol content and total flavonoid content of chloroform layer were higher in each of 201.98±10.31 mg/mL and 13.55±1.33 mg/mL, DPPH and ABTs scavenging activity were showed the lowest IC50 values in each 183.95±2.67 ㎍/mL and 10.00±0.33 ㎍/mL.
α?glucosidase inhibition activity of chloroform layer was 45.65±0.73 ㎍/mL, exhibited excellent α-glucosidase inhibition than 267.07±3.73㎍/mL of acarbose.
Analyzed by NMR and LC/MS of the two compounds obtained from chloroform fractions of Maackia amurensis bark, compound 1 and compound 2 were confirmed by each of Formononetin and Tectorigenin.
Researching the α-glucosidase inhibition of a separated compound, α-glucosidase inhibition activity of Formononetin was 17.13±0.50 μM, showed IC50 values in the low concentration greater than 319.8 ± 16.57 μM of acarbose.
The results of this study, Maackia amurensis bark contained Formononetin representing the α-Glucosidase inhibition activity. So the development potential of new diabetes is expected in the future.
Bark extracts of Maackia amurensis were prepared using 70% methanol. Methanol extracts were fractionated to hexane, chloroform, ethyl acetate, butyl alcohol, water layer and investigated the physiological activity, α-glucosidase inhibitory and structural analysis.
The polyphenol contents of 70% methanol extracts from Maackia amurensis bark was higher by 14.44±0.44 mg/g, IC50 values of electron donating ability and α-glucosidase inhibitory were lower in each of 194.15±2.96 ㎍/mL and 322.19±2.63 ㎍/mL.
The total polyphenol content and total flavonoid content of chloroform layer were higher in each of 201.98±10.31 mg/mL and 13.55±1.33 mg/mL, DPPH and ABTs scavenging activity were showed the lowest IC50 values in each 183.95±2.67 ㎍/mL and 10.00±0.33 ㎍/mL.
α?glucosidase inhibition activity of chloroform layer was 45.65±0.73 ㎍/mL, exhibited excellent α-glucosidase inhibition than 267.07±3.73㎍/mL of acarbose.
Analyzed by NMR and LC/MS of the two compounds obtained from chloroform fractions of Maackia amurensis bark, compound 1 and compound 2 were confirmed by each of Formononetin and Tectorigenin.
Researching the α-glucosidase inhibition of a separated compound, α-glucosidase inhibition activity of Formononetin was 17.13±0.50 μM, showed IC50 values in the low concentration greater than 319.8 ± 16.57 μM of acarbose.
The results of this study, Maackia amurensis bark contained Formononetin representing the α-Glucosidase inhibition activity. So the development potential of new diabetes is expected in the future.
목차
Ⅰ. 서론 1Ⅱ. 재료 및 방법 42.1 실험재료 42.1.1 재료 42.1.2 시약 및 기기 42.1.2.1 시약 42.1.2.2 기기 42.2 시료조제 52.2.1 시료추출조건 검색 52.2.2 추출 및 분획 52.3 생리 활성 검증 72.3.1 Total Polyphenol 함량 72.3.2 Total Flavonoid 함량 72.3.3 Electron donating ability 측정 72.3.4 ABTs radical cation decolorization 측정 82.3.5 α-Glucosidase 저해 활성 측정 82.4 다릅나무 수피로부터 활성물질의 분리 92.4.1 Prep LC를 이용한 Chloroform 분획으로부터의 활성물질 분리 92.4.2 화합물 1의 분리 102.4.3 화합물 2의 분리 102.4.4 분리한 화합물 1, 2의 구조분석 112.4.4.1 HPLC-PDA 112.4.4.2 Q-TOF-MS 122.4.4.3 NMR 132.5 통계분석 13Ⅲ. 결과 및 고찰 143.1 시료추출 조건검색 143.2 추출 및 분획 173.3 분획물의 생리 활성 검증 183.3.1 Total polyphenol 함량 183.3.2 Total flavonoid 함량 193.3.3 Electron donating ability 측정 203.3.4 ABTs radical cation decolorization 측정 213.3.5 α-Glucosidase 저해 활성 측정 223.4 분리한 활성물질의 구조분석 243.4.1 화합물 1의 HPLC 분석 243.4.2 화합물 1의 Q-TOF-MS 분석 253.4.3 화합물 1의 NMR 263.4.4 화합물 2의 HPLC 분석 293.4.5 화합물 2의 Q-TOF-MS 분석 303.4.6 화합물 2의 NMR 313.5 분리한 화합물에 대한 α-Glucosidase 저해 활성 측정 35Ⅳ. 적 요 36참고문헌 37Abstract 43
최근 본 자료
댓글(0)
0