목차

Abstract ⅰ
요약문 ⅱ
Contents ⅳ
List of Tables ⅶ
List of Figures ⅷ
1. Introduction 1
2. Material and Methods 3
2.1 Artificial soil 3
2.1.1 Industrial sand 3
2.1.2 Kaolin clay 3
2.1.3 Sphagnum peat 3
2.2 Artificial acid mine drainage 4
2.3 Microorganisms and Culture 4
2.3.1 Isolation of indigenous soil microorganisms 4
2.3.2 Myxococcus xanthus 6
2.4 TOC of sphagnum peat 6
2.5 Method of microbial neutralization experiment 7
2.5.1 Microbiological neutralization with artificial mine drainage 7
2.5.2 Neutralization potential as a function of artificial soil quantity 8
2.5.3 Neutralization potential as a function of more than 50 g of artificial soil quantity 9
2.5.4 Neutralization potential as a function of peat moss quantity 10
2.5.5 Neutralization potential as a function of artificial mine soil quantity 11
2.5.6 Neutralization potential as a function of concentration of microorganism 12
2.6 Analytical methods 13
2.6.1 The 16S rRNA gene cloning for the indigenous soil microorganisms 13
2.6.2 pH 13
2.6.3 Acidity 13
2.6.4 NH4+ ion concentration 14
2.6.5 Microbial activity 14
3. Results and Discussion 15
3.1 Identification of bacteria species by 16S rRNA gene analysis 15
3.1.1 Indigenous microorganisms A 15
3.1.2 Indigenous microorganisms B 17
3.2 TOC of peat moss 19
3.3 Microbiological experiment of neutralization potential in accordance with artificial mine drainage 19
3.4 Experiment of neutralization potential in accordance with amount of artificial soil 21
3.4.1 Indigenous microorganisms A 21
3.4.2 Indigenous microorganisms B 23
3.4.3 M. xanthus 24
3.5 Experiment of neutralization potential in accordance with amount of more than 50g of artificial mine soil 26
3.5.1 Indigenous microorganisms A 26
3.5.2 Indigenous microorganisms B 28
3.6 Experiment of neutralization potential in accordance with amount of peat moss 30
3.6.1 Indigenous microorganisms A 30
3.6.2 Indigenous microorganisms B 33
3.7 Experiment of neutralization potential in accordance with amount of artificial mine soil 34
3.7.1 Indigenous microorganisms A 35
3.7.2 Indigenous microorganisms B 36
3.8 Experiment of neutralization potential in accordance with concentration of microorganisms 38
3.8.1 Indigenous microorganisms A (5 %) 39
3.8.2 Indigenous microorganisms A (10 %) 41
3.8.1 Indigenous microorganisms B (5 %) 42
3.8.2 Indigenous microorganisms B (10 %) 44
3.9 Experimental result of correlation between pH and microbial activity 47
3.10 Experimental result of correlation between pH and NH4+ 49
4. Conclusion 51
References 53