목차

목 차
표목차 ⅲ
그림목차 ⅳ
ABSTRACT ⅶ
Ⅰ. 서 론 1
Ⅱ. 연구방법 4
1. 재료 및 시약 4
2. 추출물의 제조 4
1)유기용매추출 5
2)초임계추출 6
3. 항산화 효과 분석 7
1) 총 폴리페놀 함량 측정 7
2) 총 플라보노이드 함량 측정 7
3) DPPH라디칼 소거능 측정 7
4) 아질산염 소거능 측정 8
4. 인간모유두세포에 대한 in vitro 세포 독성 평가 9
1) 천연추출물처리 9
2) 세포배양 9
3) MTT assay 9
5. Western blotting을 통한 성장인자 발현에 미치는 영향 11
6.통계처리 12
Ⅲ. 연구결과 13
1. 생약제재 추출물의 수율 13
2. 항산화 효과 14
1) 총 폴리페놀 함량 14
2) 총 플라보노이드 함량 16
3) DPPH 라디칼 소거능 18
4) 아질산염 소거능 20
3. 인간모유두세포에 대한 in vitro 세포 독성 평가 22
1) 황칠나무 22
2) 찔레뿌리 23
3) 황칠 추출물과 찔레뿌리 추출물위 1:1 혼합물 24
4.Western blot 25
1) PDGF-B 발현 촉진 효과 25
2) IGF 발현 촉진 효과 26
3) VEGF 발현 촉진 효과 27
4) KGF 발현 촉진 효과 28
5) hGH 발현 촉진 효과 29
6) EGF 발현 촉진 효과 30
Ⅳ. 고찰 31
Ⅴ.결론 38
참고문헌 40
표 목차
Table 1. Extraction conditions for supercritical extraction group·················7·
Table 2. Extracted yield in the water, ethanolic, methanolic, ethanol-sonication, methanol-sonication, and super critical extracts from Dendropanax morbifera and Rosa multiflora················································15
Table 3. Total polyphenols content of Dendropanax morbifera extracts ··········16
Table 4. Total polyphenols content of Rosa multiflora root extracts··············17
Table 5. Total flavonoids content of Dendropanax morbifera extracts·············18
Table 6. Total flavonoids content of Rosa multiflora root extracts················19
그림 목차
Figure 1. Principle of cytotoxicity evaluation test through MTT assay analysis.
···················································································9
Figure 2. DPPH radical scavenging activity of Dendropanax morbifera extracts. Con, control (vehicle) as negative control group; AA, ascorbic acid (1
mg/ml) as positive control group; DMWE, Dendropanax morbifera water extracts; DMEE, Dendropanax morbifera ethanolic extracts; DMME, Dendropanax morbifera methanolic extracts; DMESE, Dendropanax morbifera ethanolic sonication extracts; DMMSE, Dendropanax morbifera methanolic sonication extracts; DMSE, Dendropanax morbifera super critical extracts.·····························20
Figure 3. DPPH radical scavenging activity of Rosa multiflora root extracts. Con, control (vehicle) as negative control group; AA, ascorbic acid (1 mg/ml) as positive control group; RMWE, Rosa multiflora water extracts; RMEE, Rosa multiflora ethanolic extracts; RMME, Rosa multiflora methanolic extracts; RMESE, Rosa multiflora ethanolic sonication extracts; RMMSE, Rosa multiflora methanolic sonication extracts; RMSE, Rosa multiflora super critical extracts. determinations·································································21
Figure 4. The nitrite-scavenging activity of Dendropanax morbifera extracts. Con, control (vehicle) as negative control group; AA, ascorbic acid (1 mg/ml) as positive control group; DMWE, Dendropanax morbifera water extracts; DMEE, Dendropanax morbifera ethanolic extracts; DMME, Dendropanax morbifera methanolic extracts; DMESE, Dendropanax morbifera ethanolic sonication extracts; DMMSE, Dendropanax morbifera methanolic sonication extracts; DMSE, Dendropanax morbifera super critical extracts. ····························22
Figure 5. The nitrite-scavenging activity of Rosa multiflora root extracts. Con, control (vehicle) as negative control group; AA, ascorbic acid (1 mg/ml) as positive control group; RMWE, Rosa multiflora water extracts; RMEE, Rosa multiflora ethanolic extracts; RMME, Rosa multiflora methanolic extracts; RMESE, Rosa multiflora ethanolic sonication extracts; RMMSE, Rosa multiflora methanolic sonication extracts; RMSE, Rosa multiflora super critical extracts. ·················23
Figure 6. Effect of cytotoxicity from methanol-sonication extracts from Dendropanax morbifera extracts on the cell viability of Human follicle dermal papilla cells.··························································24
Figure 7. Effect of cytotoxicity from methanol-sonication extracts from Rosa multiflora extracts on the cell viability of Human follicle dermal papilla cells.···································································25
Figure 8. Effect of cytotoxicity from methanol-sonication extracts from Dendropanax morbifera from methanol-sonication extracts Rosa multiflora extracts on the cell viability of Human follicle dermal papilla cells. ··································································26
Figure 9. Effect of promote revelation from PDGF-B from Dendropanax morbifera and Rosa multiflora················································27
Figure 10. Effect of promote revelation from IGF from Dendropanax morbifera and Rosa multiflora.···························································28
Figure 11. Effect of promote revelation from VEGF from Dendropanax morbifera and Rosa multiflora.···························································29
Figure 12. Effect of promote revelation from KGF from Dendropanax morbifera and Rosa multiflora.···························································30
Figure 13. Effect of promote revelation from hGH from Dendropanax morbifera and Rosa multiflora (RM).····················································31
Figure 14. Effect of promote revelation from EGF from Dendropanax morbifera and Rosa multiflora .··························································32