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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김인호
- 발행연도
- 2016
- 저작권
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The moment analyses of (R)-(+)-limonene and lysozyme were implemented using chromatograms that were obtained with the pulse input method from an octadecyl silica(ODS) column in reversed-phase high performance liquid chromatography(RP-HPLC) and weak cation exchange column in cation exchange high performance liquid chromatography(CE-HPLC). The particle diameters of the stationary phase were the same as 5. Mobile phases were the mixture of ethanol and water in RP-HPLC and sodium phosphate buffer in CE-HPLC. Experiments were conducted by varying flow rate, volume ratio of the mobile phase, and solute concentration.
The general rate(GR) model was employed to calculate the first moment and the second moment. By plotting vs. , equilibrium constants(K) were obtained as results of first moment analyses. In the second moment analyses, two coefficients for moment analysis, molecular diffusivity and external mass transfer coefficient, were estimated by the Wilke-Chang and Wilson-Geankoplis equations, respectively. The intra particle diffusivity was obtained by comparing theoretical plate number equation with the van Deemter equation. After obtaining results of two moment analyses results based on the experimental elution curve, van Deemter plots were drawn and van Deemter coefficients were compared each other in order to investigate the effects of , , and on Height Equivalent to a Theoretical Plate(HETP, ). In two HPLC modes, the value of intra-particle diffusion() was the most dominant factor contributing to HETP, with a linear relationship, while external mass transfer() was negligible factor.
The general rate(GR) model was employed to calculate the first moment and the second moment. By plotting vs. , equilibrium constants(K) were obtained as results of first moment analyses. In the second moment analyses, two coefficients for moment analysis, molecular diffusivity and external mass transfer coefficient, were estimated by the Wilke-Chang and Wilson-Geankoplis equations, respectively. The intra particle diffusivity was obtained by comparing theoretical plate number equation with the van Deemter equation. After obtaining results of two moment analyses results based on the experimental elution curve, van Deemter plots were drawn and van Deemter coefficients were compared each other in order to investigate the effects of , , and on Height Equivalent to a Theoretical Plate(HETP, ). In two HPLC modes, the value of intra-particle diffusion() was the most dominant factor contributing to HETP, with a linear relationship, while external mass transfer() was negligible factor.
목차
1. 서 론 12. 문헌조사 및 이론적 배경 72.1. (R)-(+)-limonene 72.2. Lysozyme 92.3. 액체크로마토그래피 132.4. Moment analysis(MA) 163. 실험 재료 및 방법 203.1. 역상컬럼(RP-HPLC)에서 (R)-(+)-limonene 분석 203.2. 양이온교환컬럼(CE-HPLC)에서 lysozyme 분석 233.3. General rate(GR) model을 이용한 Moment analysis(MA) 264. 결과 및 토론 324.1. RP-HPLC에서 (R)-(+)-limonene의 분석 결과 324.2. CE-HPLC에서 Lysozyme의 분석 결과 364.3. RP-HPLC에서 (R)-(+)-limonene의 MA결과 404.4. CE-HPLC에서 lysozyme의 MA결과 525. 결 론 63Nomenclature 65Greek letters 66Abstract 67References 69
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