지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김순중
- 발행연도
- 2016
- 저작권
- 세명대학교 논문은 저작권에 의해 보호받습니다.
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이 논문의 연구 히스토리 (2)
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- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Objectives
This study was designed to investigate whether the Cheongajihwang-Tang (CT) has an inhibitory effect association with oxidation or inflammation in RAW264.7 cells.
Methods
Cytotoxic activity of CT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Nitric oxide production was measured using Griess reagent system. The total phenolic contents and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the antioxidative effects of CT. Dichlorofluorescin diacetate (DCFH-DA) has been used as a substrate for measuring intracellular oxidant production.
Results
Cheongajihwang-Tang does not impair the cell viability in tested concentration. CT showed anti-oxiative effects in vitro by decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and ROS expressin in LPS induced RAW264.7 activation. CT inhibited the generation of intracellular ROS production as dose dependant manner.
Conclusions
CT has anti-oxidative effects and anti-inflammatory activities. These results indicate that CT extract has an anti-inflammatory activities via anti-oxidative effects.
This study was designed to investigate whether the Cheongajihwang-Tang (CT) has an inhibitory effect association with oxidation or inflammation in RAW264.7 cells.
Methods
Cytotoxic activity of CT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Nitric oxide production was measured using Griess reagent system. The total phenolic contents and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the antioxidative effects of CT. Dichlorofluorescin diacetate (DCFH-DA) has been used as a substrate for measuring intracellular oxidant production.
Results
Cheongajihwang-Tang does not impair the cell viability in tested concentration. CT showed anti-oxiative effects in vitro by decreasing electron donating ability, and also showed anti-inflammatory effects suppressing NO and ROS expressin in LPS induced RAW264.7 activation. CT inhibited the generation of intracellular ROS production as dose dependant manner.
Conclusions
CT has anti-oxidative effects and anti-inflammatory activities. These results indicate that CT extract has an anti-inflammatory activities via anti-oxidative effects.
목차
Ⅰ. 서론·············································································1Ⅱ. 재료 및 방법 ·································································31. 재료········································································31)약재 ···········································································32)시약 ···········································································53)세포 ···········································································52. 방법···········································································61)총 폴리페놀 함량 ····························································62)항산화 효과 ·································································73)세포배양 ······································································74)RAW 264.7 세포 독성 시험 ·············································85)NO 생성 억제효과 측정 ···················································96)DCFH-DA에 의한 intracellular ROS 측정 ·························97)통계 분석 ···································································10Ⅲ. 결과·········································································111. 총 폴리페놀 함량 ···························································112. DPPH free radical 소거능 ················································123. 세포 독성 측정 ·····························································134. Nitric oxide 측정····························································145. Intracellular ROS 생성 저해 ·············································15Ⅳ. 고찰·········································································16Ⅴ. 결론·········································································21참고문헌·······································································22Abstracts··········································································30
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