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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 이강노
- 발행연도
- 2016
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As a part of our continuing search for bioactive constituents from Korean medicinal plants, I investigated methanol extracts of the twigs of Corylopsis coreana and twigs of Ligustrum obtusifolium. The rhizodermis of C. coreana have been used in to treat cold and heat in Korean folk medicine1.1) Previous phytochemical investigations on this plant reported the isolation of phenyl propanoid derivatives, tannins, flavonoids and terpenoids2,3) and some of them exhibits antioxidant and anti-inflammatory4) effects. L. obtusifolium, known as Ibota privet, is a shrub that is widely distributed in East Asia. fruit of this plant has been used as a Korean traditional medicine for the treatment of haemostasis, diabetes, cold sweat, and hypertension. previous phytochemical investigations on L. obtusifolium reported isolation of several secoiridoid glycosides5) with lysine decreasing activity6) and anti-hyperglycaemic7) activities.
Through solvent fractionation and isolation of the methanolic extracts of the two plants, 3 bergenin derivatives, 3 flavonoids, and 4 phenolic compounds from the twigs of C. coreana and 8 secoiridoid glycosides and 5 lignans from the twigs of L. obtusifolium were isolated. the structures of compounds from C. coreana were identified as bergenin (C-1), 6′-O-galloylbergenin (C-2), 3′-O-galloylbergenin (C-3), (-)-catechin (C-4), (-)-epicatechin (C-5), (-)-epicatechin-3-O-galloyl ester (C-6), 4-methoxy-3,-5-dihydroxybenzoic acid (C-7), gallic acid (C-8), 2,4,6-trimethoxyphenol-1-O-β-D-glucopyranoside (C-9), and 2,4,6-trimethoxyphenol-1-O-β-D-(6-O-galloyl)-glucopyranoside (C-10), using spectroscopic data including NMR, MS, UV, IR. Structures of compounds from L. obtusifolium were determined to be Obtusifoliside (L-1), Oleoside11-methylester (L-2), Oleside-7,11-dimethylester (L-3), Ligstroside (L-4), Oleuropein (L-5), (2''''R)-2''''-Methoxyoleuropein (L-6), 10-hydroxyoleuropein (L-7), neonuezhenide (L-8), (-)-Olivil (L-9), (+)-Pinoresinol mono-methxy-O-β-D-glucoside (L-10), phillyrin (L-11), (+)-pinoresinol-β-D-glucoside (L-12), (+)-Syringaresinol-O-β-D-glucopyranoside (L-13), by, NMR, MS, UV, IR, and chemical reaction. among these isolates, compound L-1 was newly isolated from the nature.
All the isolates (C-1 - C-10, L-1 - L13) were tested for their cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cell lines in vitro using the SRB bioassay.
Through solvent fractionation and isolation of the methanolic extracts of the two plants, 3 bergenin derivatives, 3 flavonoids, and 4 phenolic compounds from the twigs of C. coreana and 8 secoiridoid glycosides and 5 lignans from the twigs of L. obtusifolium were isolated. the structures of compounds from C. coreana were identified as bergenin (C-1), 6′-O-galloylbergenin (C-2), 3′-O-galloylbergenin (C-3), (-)-catechin (C-4), (-)-epicatechin (C-5), (-)-epicatechin-3-O-galloyl ester (C-6), 4-methoxy-3,-5-dihydroxybenzoic acid (C-7), gallic acid (C-8), 2,4,6-trimethoxyphenol-1-O-β-D-glucopyranoside (C-9), and 2,4,6-trimethoxyphenol-1-O-β-D-(6-O-galloyl)-glucopyranoside (C-10), using spectroscopic data including NMR, MS, UV, IR. Structures of compounds from L. obtusifolium were determined to be Obtusifoliside (L-1), Oleoside11-methylester (L-2), Oleside-7,11-dimethylester (L-3), Ligstroside (L-4), Oleuropein (L-5), (2''''R)-2''''-Methoxyoleuropein (L-6), 10-hydroxyoleuropein (L-7), neonuezhenide (L-8), (-)-Olivil (L-9), (+)-Pinoresinol mono-methxy-O-β-D-glucoside (L-10), phillyrin (L-11), (+)-pinoresinol-β-D-glucoside (L-12), (+)-Syringaresinol-O-β-D-glucopyranoside (L-13), by, NMR, MS, UV, IR, and chemical reaction. among these isolates, compound L-1 was newly isolated from the nature.
All the isolates (C-1 - C-10, L-1 - L13) were tested for their cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cell lines in vitro using the SRB bioassay.
목차
Ⅰ. Introduction1. Corylopsis coreana and Ligustrum obtusifolium........................................1Ⅱ. MATERIALS AND METHODS1. Plats matarials.......................................................................................................51.1 C. coreana.......................................................................................................51.2 L. obutsifolium..............................................................................................52. Reagents and Equipments..................................................................................53. Isolation and purification3.1 C. coreana.......................................................................................................73.2 L. obutsifolium.............................................................................................164. Biological evaluations4.1 Cancer cell lines..........................................................................................284.2 Method............................................................................................................28Ⅲ. Results1. Structure Elucidation of Compounds isolated from C. coreana1. 1 The structure of Compound C-1.........................................................301. 2 The structure of Compound C-2.........................................................341. 3 The structure of Compound C-3.........................................................381. 4 The structure of Compound C-4.........................................................421. 5 The structure of Compound C-5.........................................................461. 6 The structure of Compound C-6.........................................................501. 7 The structure of Compound C-7.........................................................531. 8 The structure of Compound C-8.........................................................571. 9 The structure of Compound C-9.........................................................601. 10 The structure of Compound C-10.....................................................642. Structure Elucidation of Compounds isolated from L. obtusifolium2. 1 The structure of Compound L-1.........................................................682. 2 The structure of Compound L-2.........................................................752. 3 The structure of Compound L-3.........................................................792. 4 The structure of Compound L-4.........................................................832. 5 The structure of Compound L-5.........................................................872. 6 The structure of Compound L-6.........................................................912. 7 The structure of Compound L-7.........................................................952. 8 The structure of Compound L-8.........................................................992. 9 The structure of Compound L-9.......................................................1032. 10 The structure of Compound L-10...................................................1072. 11 The structure of Compound L-11...................................................1112. 12 The structure of Compound L-12...................................................1152. 13 The structure of Compound L-13...................................................1193. Cytotoxic Activities of Compounds.............................................................126Ⅳ. Conclusion............................................................................................................128Ⅴ. Refernces.................................................................................................................129국문요약.............................................................................................................................133
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