지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 박호원
- 발행연도
- 2016
- 저작권
- 강릉원주대학교 논문은 저작권에 의해 보호받습니다.
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이 논문의 연구 히스토리 (2)
2017
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Abstract
Objective:
The purpose of this study was to compare and to evaluate the cariogenic potential of commercially available bovine milks with different fat content on Streptococcus mutans biofilm using the CDC Biofilm Reactor.
Materials and Methods:
S. mutans ATCC 25175 biofilms were formed on saliva coated bovine enamel slabs using the CDC Biofilm Reactor. Slabs/biofilms were exposed three times per day to one of the following materials: commercial whole milk (fat content: 3.4%) and low fat milk (fat content: 1%). Medium pH was monitored three times per day. After 5 days, the biofilms depth was evaluated by confocal laser-scanning microscopy (CLSM). The biofilms were separated from slabs to evaluate the number of viable bacteria. Enamel slab’s demineralization was assessed by measuring surface microhardness before and after the experiment.
Results:
For microhardness and viable cells assessment, no significant difference was detected between whole milk and low fat milk group. Data from biofilm depth evaluated by confocal laser-scanning microscopy showed similar biofilm depth. Two groups showed a similar pattern of medium pH change.
Conclusions:
The results showed that commercially available whole milk and low fat milk have similar cariogenicity. Fat content in the milks seemed to have no influence on the cariogenicity of the S. mutans biofilm.
Objective:
The purpose of this study was to compare and to evaluate the cariogenic potential of commercially available bovine milks with different fat content on Streptococcus mutans biofilm using the CDC Biofilm Reactor.
Materials and Methods:
S. mutans ATCC 25175 biofilms were formed on saliva coated bovine enamel slabs using the CDC Biofilm Reactor. Slabs/biofilms were exposed three times per day to one of the following materials: commercial whole milk (fat content: 3.4%) and low fat milk (fat content: 1%). Medium pH was monitored three times per day. After 5 days, the biofilms depth was evaluated by confocal laser-scanning microscopy (CLSM). The biofilms were separated from slabs to evaluate the number of viable bacteria. Enamel slab’s demineralization was assessed by measuring surface microhardness before and after the experiment.
Results:
For microhardness and viable cells assessment, no significant difference was detected between whole milk and low fat milk group. Data from biofilm depth evaluated by confocal laser-scanning microscopy showed similar biofilm depth. Two groups showed a similar pattern of medium pH change.
Conclusions:
The results showed that commercially available whole milk and low fat milk have similar cariogenicity. Fat content in the milks seemed to have no influence on the cariogenicity of the S. mutans biofilm.
목차
목 차Ⅰ. 서론 ----------------------------------------------------1Ⅱ. 연구재료 및 방법 -----------------------------------------3Ⅲ. 연구 결과 -----------------------------------------------10Ⅳ. 총괄 및 고찰 ------------------------------------------16Ⅴ. 결론 --------------------------------------------------20Ⅵ. 참고문헌 -----------------------------------------------21Ⅶ. 영문초록 -----------------------------------------------24
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