목차

CHAPTER 1. INTRODUCTION 1
CHAPTER 2. THEORETICAL BACKGROUND 4
2-1. Gene therapy 4
2-1-1. Gene therapy using viral vector 5
2-1-1-1. Advantage and disadvantage 5
2-1-2. Gene therapy using non-viral vector 6
2-1-2-1. Advantage and disadvantage 6
2-2. Polymer as gene delivery carrier 7
2-2-1. Urethane for biomedical applications 7
2-2-2. L-tyrosine 8
2-2-3. L-tyrosine polyurethane (LTU) 9
2-2-4. Linear polyethyleneimine (LPEI) 10
2-2-4-1. Cytotoxicity of PEI 10
2-2-4-2. PEI-DNA complex by electorstatic attarction 10
2-2-4-3. Proton sponge effect of LPEI 11
2-2-5. LTU nanoparticles double emulsion method 12
2-3. Biodegradable nanoparticles for drug or gene delivery 13
CHAPTER 3. EXPERIMENTAL 14
3-1. Materials 14
3-2. Measurement 16
3-2-1. Spectroscopic measurement 16
3-2-2. Microscopic measurement 16
3-3. Preparation of LTU nanoparticles for gene delivery application 17
3-3-1. Syntehsis of LTU 17
3-3-1-1. Tyrosine hexyl ester (TH) 17
3-3-1-2. Desaminotyrosyl tyrosine hexyl ester (DTH) 19
3-3-1-3. L-tyrosine polyurethane (LTU) 21
3-3-2. Preaparation of FITC-BSA LTU nanoparticles (Double-emulsion) 23
3-3-3. Preaparation of DNA-LPEI LTU nanoparticles (Double-emulsion) 23
3-3-4. Cell uptake study of FITC-BSA LTU NPs 26
3-3-5. Transfection study of DNA-LPEI LTU NPs 26
CHAPTER 4. RESULTS AND DISCUSSION 27
4-1. Characterization of TH, DTH, LTU 27
4-2. FITC-BSA LTU nanoparticles 29
4-2-1. Diameter distribution and encapsulation 29
4-2-2. In vitro test of FITC-BSA LTU NPs 31
4-3. DNA-LPEI LTU nanoparticles 33
4-3-1. Diameter distribution 33
4-3-2. Encapsulation of pDNA confirmation 34
4-3-3. Determination of DNA loading and release of DNA-LPEI complex 36
4-3-4. Cumulative release test of DNA-LPEI LTU NPs 38
4-3-5. Transfection and cytotoxicity studies of DNA-LPEI LTU NPs 39
CHAPTER 5. CONCLUSIONS 43
REFERENCES 44
ABSTRACT (KOREAN) 46