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이 논문의 연구 히스토리 (4)
2017
치자(Gardenia jasminoides Ellis) 과피의 용매별 추출물의 Flavonoid 함량 및 항산화 활성 비교
진동혁
,
오다영
,
이영근
외 2명
한국환경과학회지
2017.08
학술저널
남해 치자(gradenia jasminoides ellis) 열매의 추출 용매에 따른 생리활성 및 항산화 효과
진동혁
식품공학과
2017.01
학위논문
남해산 치자(Gardenia jasminoides Ellis fructus) 씨 추출물의 항산화 능 및 생리활성에 미치는 영향
진동혁
,
김한수
,
이영근
외 1명
한국유화학회지
2017.01
학술저널
치자(Gardenia jasminoides Ellis fructus) 껍질 추출물의 항산화 활성
진동혁
,
오다영
,
김한수
한국유화학회지
2017.01
학술저널
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The purpose of this study was to measure the bioactivity and antioxidant activity of peel and seed from Gardenia jasminoides Ellis fructus (GJE). GJE have been known to contain functional materials such as crocin, crocetin, geniposide, gardenosid, geniposidic acid, iridoid glycosides etc. We were separated into GJE seeds and peel. After that, we determined color assessment, β-carotene, lycopene, chlorophyll a, chlorophyll b, proanthocyanidin, anthocyanin, tannin, phytic acid, total carotene. GJE seeds and peel were extracted by 70% methanol, 70% ethanol, chloroform:methanol (CM, 2:1, v/v), ethyl acetate (EA), n-butanol and distilled water (DW) of six solvents. To investigate by the solvent extract of total phenol, flavonoid content and value as a functional food ingredient of GJE through antioxidant activity (DPPH radical scavenging activity, ABTS radical scavenging activity, superoxide dismutase like ability, hydroxyl radical scavenging activity, ferrous ion-chelating capacity, nitric oxide radical scavenging activity, nitrite scavenging activity, antioxidant power by β-carotene bleaching assay), reducing power (ferric reducing antioxidant power, reducing power), inhibition of lipid peroxidation measurements were performed. GJE result in higher peel measuring the color assessment of the peel and seeds from the seed significantly higher L*, b*, C*, H° any value ever. The a* values were not significantly different (p<0.05).
Peel and seed of GJE the physiologically active substance content of β-carotene (0.152, 0.336 mg/ 100 g DW), proanthocyanidin (48.165, 70.035 mg CE/g DW), chlorophyll a (0.068, 0.059 mg/100 g DW), chlorophyll b (0.122, 0.121 mg/ 100 g DW), lycopene (0.151, 0.126 mg/100 g DW), anthocyanin (3.519, 2.201 mg/100 g DW), tannin (1.681, 1.517 mg CE/g DW), phytic acid (4.966, 1.157 mg PAE/g DW), total carotene (1.498, 1.316 mg BCE/g DW). In the results, β-carotene and proanthocyanidin contents of seeds showed significantly higher than the peel. Chlorophyll a, lycopene, anthocyanin, tannin, phytic acid, total carotene content of peel were observed to be higher than seeds, chlorophyll b contents were no significantly different (p<0.05). Peel extract yield of GJE were DW (39.87%), 70$ methanol (36.26%), 70%, ethanol (26.95%), CM (24.44%), n-butanol (5.13%), EA (2.88%). Seed extract yield were DW (36.61%), CM (36.39%), 70% methanol (30.10%), 70% ethanol (27.32%), n-butanol (26.23%), EA (20.40%). Solvent extraction antioxidant activity of increasing concentrations (0.2, 0.4, 0.6 mg/mL) was significantly increased (p<0.05). GJE extracts showed lower activity than positive control (ascorbic acid, BHA, EDTA, trolox). When compared with the same solvent extraction of the peel and seeds mainly peel showed higher activity than seeds. Total phenol content (mg CAE/g) of GJE peel extracts were CM (39.74), n-butanol (31.54), 70% ethanol (28.73), EA (26.59), 70% methanol (24.13), DW (18.61) order was shown, the seeds extract were observed in the CM (32.50), 70% ethanol (30.09), 70% methanol (28.60), DW (21.98), n-butanol (11.07), EA (10.19) order. Flavonoid content (mg QE/g) of GJE peel extracts were CM (0.664), n-butanol (0.550), 70% ethanol (0.526), 70% methanol (0.416), DW (0.225), EA (0.212), similar to the total phenol in the peel. Flavonoid content of seeds was confirmed in 70% ethanol (0.830), 70% methanol (0.799), CM (0.752), DW (0.565), EA (0.117), n-butanol (0.105). These results, by a solvent of peel were found that the relationship with the increase of total phenol and flavonoid content increased physiological activity of the antioxidant activity, reducing power, lipid peroxidation inhibition assay. The antioxidant activity of the extract from the other
except for the EA extract on peel was observed at a high level. The β-carotene was observed in 70% methanol extract, the inhibition discoloring analysis differently from other analysis results lower than DW extract. Antioxidant activity of the extract from the seeds were also similar to the results of total phenol and flavonoid content, in other extracts, except for EA, n-butanol extracts showed high activity. The results suggest that GJE seeds and peel both can be used as nutraceutical foods and natural antioxidant.
Peel and seed of GJE the physiologically active substance content of β-carotene (0.152, 0.336 mg/ 100 g DW), proanthocyanidin (48.165, 70.035 mg CE/g DW), chlorophyll a (0.068, 0.059 mg/100 g DW), chlorophyll b (0.122, 0.121 mg/ 100 g DW), lycopene (0.151, 0.126 mg/100 g DW), anthocyanin (3.519, 2.201 mg/100 g DW), tannin (1.681, 1.517 mg CE/g DW), phytic acid (4.966, 1.157 mg PAE/g DW), total carotene (1.498, 1.316 mg BCE/g DW). In the results, β-carotene and proanthocyanidin contents of seeds showed significantly higher than the peel. Chlorophyll a, lycopene, anthocyanin, tannin, phytic acid, total carotene content of peel were observed to be higher than seeds, chlorophyll b contents were no significantly different (p<0.05). Peel extract yield of GJE were DW (39.87%), 70$ methanol (36.26%), 70%, ethanol (26.95%), CM (24.44%), n-butanol (5.13%), EA (2.88%). Seed extract yield were DW (36.61%), CM (36.39%), 70% methanol (30.10%), 70% ethanol (27.32%), n-butanol (26.23%), EA (20.40%). Solvent extraction antioxidant activity of increasing concentrations (0.2, 0.4, 0.6 mg/mL) was significantly increased (p<0.05). GJE extracts showed lower activity than positive control (ascorbic acid, BHA, EDTA, trolox). When compared with the same solvent extraction of the peel and seeds mainly peel showed higher activity than seeds. Total phenol content (mg CAE/g) of GJE peel extracts were CM (39.74), n-butanol (31.54), 70% ethanol (28.73), EA (26.59), 70% methanol (24.13), DW (18.61) order was shown, the seeds extract were observed in the CM (32.50), 70% ethanol (30.09), 70% methanol (28.60), DW (21.98), n-butanol (11.07), EA (10.19) order. Flavonoid content (mg QE/g) of GJE peel extracts were CM (0.664), n-butanol (0.550), 70% ethanol (0.526), 70% methanol (0.416), DW (0.225), EA (0.212), similar to the total phenol in the peel. Flavonoid content of seeds was confirmed in 70% ethanol (0.830), 70% methanol (0.799), CM (0.752), DW (0.565), EA (0.117), n-butanol (0.105). These results, by a solvent of peel were found that the relationship with the increase of total phenol and flavonoid content increased physiological activity of the antioxidant activity, reducing power, lipid peroxidation inhibition assay. The antioxidant activity of the extract from the other
except for the EA extract on peel was observed at a high level. The β-carotene was observed in 70% methanol extract, the inhibition discoloring analysis differently from other analysis results lower than DW extract. Antioxidant activity of the extract from the seeds were also similar to the results of total phenol and flavonoid content, in other extracts, except for EA, n-butanol extracts showed high activity. The results suggest that GJE seeds and peel both can be used as nutraceutical foods and natural antioxidant.
목차
Table legends ................................................................................................................................. ivScheme & Figure legends .........................................................................................................v1. 서론 ............................................................................................................................................... 12. 재료 및 방법 ............................................................................................................................. 122.1. 실험 재료 ........................................................................................................................... 122.2. 시료의 추출 ....................................................................................................................... 122.3. Bioactive compounds determination ................................................................. 142.3.1. 색도 측정 ................................................................................................................... 142.3.2. β-carotene, lycopene 및 chlorophyll a, b 함량 측정 ........................ 142.3.3. Proanthocyanidin 함량 측정 ........................................................................... 152.3.4. Anthocyanin 함량 측정 ...................................................................................... 162.3.5. Tannin 함량 측정 .................................................................................................. 172.3.6. Phytic acid 함량 측정.......................................................................................... 182.3.7. Total carotenoid 함량 측정 ............................................................................. 192.4. Antioxidant activity determination ..................................................................... 202.4.1. Total phenol 함량 측정...................................................................................... 202.4.2. Flavonoid 함량 측정 ............................................................................................ 202.4.3. DPPH radical scavenging activity 측정 ..................................................... 212.4.4. ABTS radical scavenging activity 측정 ...................................................... 222.4.5. Superoxide dismutase (SOD) like ability 측정 ...................................... 232.4.6. Hydroxyl radical (OH-) scavenging activity 측정 ................................ 242.4.7. Ferric reducing antioxidant power (FRAP) 측정 .................................. 252.4.8. Ferrous ion-chelating capacity 측정 ........................................................... 262.4.9. Reducing power 측정 ......................................................................................... 272.4.10. Nitric oxide (NO) radical scavenging activity 측정 ......................... 282.4.11. Nitrite (NO2) scavenging activity 측정 ................................................... 292.4.12. β-carotene bleaching 을 이용한 antioxidant activity 측정................. 302.4.13. Lipid peroxidation inhibition activity 측정 .......................................... 312.5. 통계 처리 ........................................................................................................................... 323. 결과 및 고찰 ............................................................................................................................. 333.1. Bioactive compounds .................................................................................................. 333.1.1. 색도 .............................................................................................................................. 333.1.2. β-carotene, lycopene 및 chlorophyll a, b 함량 ................................... 353.1.3. Proanthocyanidin 함량 ...................................................................................... 383.1.4. Anthocyanin 함량 ................................................................................................. 413.1.5. Tannin 함량 .............................................................................................................. 433.1.6. Phytic acid 함량 ..................................................................................................... 453.1.7. Total carotene 함량 ............................................................................................. 483.2. Antioxidant activity ...................................................................................................... 503.2.1. 수 율 ............................................................................................................................ 503.2.2. Total phenol 함량 ................................................................................................. 533.2.3. Flavonoid 함량........................................................................................................ 583.2.4. DPPH radical scavenging activity ................................................................ 633.2.5. ABTS radical scavenging activity ................................................................. 693.2.6. Superoxide dismutase (SOD) like ability ................................................. 733.2.7. Hydroxyl radical (OH-) scavenging activity ........................................... 773.2.8. Ferric reducing antioxidant power (FRAP) ............................................. 813.2.9. Ferrous ion-chelating capacity ...................................................................... 863.2.10. Reducing power .................................................................................................. 913.2.11. Nitric oxide (NO) radical scavenging activity..................................... 963.2.12. Nitrite (NO2) scavenging activity ........................................................... 1003.2.13. β-carotene bleaching 을 이용한 antioxidant activity .................. 1043.2.14. Lipid peroxidation inhibition activity ................................................... 1084. 결 론 ........................................................................................................ 112참 고 문 헌 ................................................................................................ 118Abstract .......................................................................................................... 148감사의 글 ................................................................................................................... 151
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