This study intends to analyze the contents of rutin, procyanidin B3, quercetin, kaempferol, known to have antioxidant, anti-inflammatory and anti-carcinogenic effects, among the polyphenol type contained in the grape pruning stem extracts (GPSE), utilizing grape stems being discarded after harvest, measure the effects on the skin moisture, inhibition of skin cell proliferation, anti-inflammatory on the damaged skin of a HR-1 hairless mice induced with UVB, and verify the applicability as a material for functional food and functional cosmetics. The polyphenol was extracted from grape pruning stem with 80% EtOH, and was used while storing at –20℃, after filtering, concentrating and freeze-drying. The contents of an active ingredient of GPSE was analyzed using HPLC. In order to induce skin damage, UVB was irradiated on the test animal, TEWL assay was performed to verify the efficacy of the GPSE, and immunohistochemical stain was conducted to measure the H&E staining on the skin tissue and COX-2 protein expression. From 53 kg of grape pruning stem specimen, 2.34 kg of EtOH fraction extracts were extracted to achieve a 4.42% of yield ratio. The analysis results of active ingredients showed 0.28 mg/g of procyanidin B3, 12.81 mg/g of rutin, 0.51 mg/g of quercetin and 8.24 mg/g of kaempferol. The TEWL assay results showed moisturizing effects on groups applied with GPSE and serum base compounds (concentration of 125 mg/kg ~ 2,000 mg/kg) as compared to the control group (p<0.05). The results of verifying the photoprotection effects through the skin proliferation control through of GPSE showed similar result to suncream was achieved at the GPSE concentration of 2,000 mg/kg on the epidermis (p<0.05). Also, a skin proliferation control on the skin of the HR-1 hairless mice damaged by UVB was confirmed by the thickness measurement results of the dermis, showing the thickness of 800 μm for the group irradiated with UVB on all GPSE concentration of 2,000 mg/kg~125 mg/kg, as compared to 580 μm for the group applied with suncream and less than 600 μm at all GPSE concentration of 2,000 mg/kg~125 mg/kg (p<0.05). In order to verify the anti-inflammation on the skin of HR-1 hairless mice damaged by the UVB and to observe the COX-2 protein expression, immunohistochemical stain was conducted. The results showed anti-inflammatory effects on all groups applied with GPSE as compared to the control group irradiated with UVB, but at the GPSE concentration of 1,000 mg/kg, a lower COX-2 protein expression at 8%, lower than the 22% of suncream, was observed to achieve an excellent anti-inflammatory effect (p<0.05). After UVB irradiation on the dermis, to confirm the degree of inhibition of fibroblast damage and collagen synthesis, we examined the protein expression of MMP-9 with immunohistochemical stain. The results of this study confirmed the existence of active polyphenol type, such as rutin, kaempferol, querocetin and procyanidin B3, within the GPSE, and GPSE has improvement effects on moisturizing effects, skin proliferation control effect, inflammatory control effect and improvement effects on the skin barrier function through UV ray damage. GPSE is a functional ingredient with a potential for skin protection effects, and has high utilization as an ingredient for functional food and functional cosmetics.