The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. This study was conducted to investigate the antimicrobial susceptibility of Staphylococcus pseudintermedius(S. pseudintermedius) isolated from dogs and cats. Of the 150 specimens, 35(23.3%) S. pseudintermedius isolates were identified by MALDI-TOF MS(Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy). Methicillin-resistant S. pseudintermedius(MRSP) were detected 5 of 35 S. pseudintermedius isolates(14.3%). In antimicrobial agents susceptibility test, almost of strains were susceptible to amikacin(100%), cephalothin(100%), vancomycin(100%), amoxicillin(85.7%), oxacillin(85.7%), enrofloxacin(82.8%), chloramphenicol(80%) and were resistant to penicillin(71.4%), tetracycline(48.6%), ampicillin(37.1%), kanamycin, erythromycin(31.4%) respectively.