목차

Table of Contents
Table of Contents .............................................................................................I
List of Figures .................................................................................................Ⅲ
Abstract (in Korean) .......................................................................................Ⅳ
Abstract .............................................................................................................Ⅴ
Chapter 1. Introduction ..................................................................................1
Chapter 2. Experimental section ....................................................................6
2.1. Materials ................................................................................................6
2.1.1. Materials ..........................................................................................6
2.2. Synthesis method .................................................................................6
2.2.1. Synthesis of Si-NH2 .......................................................................6
2.2.2. Synthesis of Si-alkyne ...................................................................6
2.2.3. Peptide synthesis ...........................................................................7
2.2.4. Intramolecular disulfide bond formation ........................................7
2.2.5. Synthesis of silica nanoparticles with peptide gatekeepers ............7
2.2.6. PEGylation of MSNs ......................................................................8
2.3. Experimental method and equipment .................................................8
2.3.1 Transmission electron microscopy .................................................8
2.3.2. Fluorescence measurements .........................................................8
2.3.3. Fourier transform infrared spectroscopy .......................................9
2.3.4. Zeta-potential analysis ...................................................................9
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2.3.5 Cell lines and culture condition .....................................................9
2.3.6. Antibodies .......................................................................................9
2.3.7. Confocal laser scanning microscopy to assess cellular uptake
of Si-cA6-PEG and intracellular release ...........................................9
2.3.8. Analysis of localization of DOX released from Si-cA6-PEG by
confocal laser scanning microscopy ..................................................9
2.3.9. TUNEL assay ...............................................................................10
2.3.10. Immunoblot analysis ..................................................................10
2.3.11. Quantification of clonogenic cell death ....................................10
2.3.12 Statistical Analysis .......................................................................11
Chapter 3. Results and discussion ..............................................................12
Chapter 4. Conclusion ...................................................................................28
References ......................................................................................................29