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논문 기본 정보

자료유형
학위논문
저자정보

강원진 (충북대학교, 충북대학교 대학원)

지도교수
김길하
발행연도
2018
저작권
충북대학교 논문은 저작권에 의해 보호받습니다.

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이 논문의 연구 히스토리 (2)

초록· 키워드

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The diamondback moth, Plutella xylostella, is a globally distributed and important economic pest, It has developed resistance to almost conventional insecticide class in the field. Chlorantraniliprole belongs to a new chemical class of insecticides acting as conformation sensitive activators of the insect ryanodine receptor (RyR). In the present study, field strain (16.3-fold resistance to chlorantraniliprole) was collected in Korea and was lab-selected with chlorantraniliprole for 8 months. As a result, the strain showed 2,157-fold resistance to chlorantraniliprole. The G4946E point mutation of the ryanodine receptor was confirmed only in the resistant strain. The expression of the RyR gene was 3.93 times higher in the resistant strain than that in the susceptible strain. Enzyme assays indicated that glutathione S-transferase (GST) and cytochrome P450 monooxygenase activities in the resistant strain was 2.4 and 1.96 times, respectively, higher compared with the susceptible strain. Its F1 progeny resulting from reciprocal crosses revealed no maternal effects and a diamide susceptible phenotype, suggesting an autosomal near recessive mode of inheritance. In addition, I surveyed insecticide susceptibility of the chlorantraniliprole - resistant strain to 13 insecticides (3 diamides, 2 synthetic pyrethroids, 2 spinosyns, 1 organophosphate, 1 oxadiazine, 1 avermectin, and 3 others), and the resistant strain exhibited highly cross resistance to flubendiamide (5,910-fold) and showed no cross resistance to spinetoram, spinosad, indoxacarb, and metaflumizone. These results can serve as an important base for guiding the use of insecticides in the field.

목차

Ⅰ. 서 론 1
Ⅱ. 연구사 3
1. 배추좀나방 3
2. 약제저항성 4
3. Chlorantraniliprole 5
4. 무독화효소 6
Ⅲ. 재료 및 방법 7
1. 실험곤충 7
2. 실험약제 7
3. 약제 감수성 평가 8
4. 효소활성 8
(1) 배추좀나방 단백질 준비 8
(2) Glutathion S-transferase (GST) 9
(3) Non-specific esterase (EST) 9
(4) Cytochrome P450 monooxygenase (P450) 9
5. Ryanodine receptor (RyR) 유전자 서열의 PCR 증폭 및 분석 10
6. Pyrosequencing 방법 10
7. Quantitative real-time PCR(qRT-PCR) 11
8. 저항성 유전 교배실험 11
9. Data analysis 12
Ⅳ. 결 과 13
1. 약제감수성 평가 13
2. Ryanodine receptor (RyR)의 유전자 서열 분석 15
3. 효소활성 18
4. Ryanodine receptor (RyR)의 유전자 발현 수준 20
5. Chlorantraniliprole 저항성 교배실험 22
6. Chlorantraniliprole 저항성 개체에 대한 교차저항성 실험 25
Ⅴ. 고 찰 27
Ⅵ. 요 약 30
Ⅶ. 인용문헌 31

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