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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 최창원
- 발행연도
- 2018
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이 논문의 연구 히스토리 (3)
2018
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This study was performed to investigate the effect of two herbal medicines, Agastachis Herba and Lysimachiae Herba on a cellular model of non-alcoholic fatty liver disease(NFLDs).
The HepG2 cells were treated with palmitic acid and were treated with variety concentrations of water extracts of Agastachis Herba(AH) or Lysimachiae Herba(LH). The lipotoxicity was assessed using EZ-cytox and the lipoapoptosis was assessed using cell death detection ELISA. The intracellular lipid was measured by staining of oil red O. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c(SREBP-1c), fatty acid synthase(FAS) and acetyl-CoA carboxylase(ACC) in HepG2 cells was measured by RT-PCR.
AH and LH increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC and FAS (SREBP-1c, ACC and FAS are factors involved in lipid synthesis). In the experiment of staining oil red O, Both extracts reduced intracellular lipid accumulation and LH''s efficacy is superior to AH''s efficacy.
According to the results, AH and LH are likely to contribute to non-alcoholic fatty liver disease, both AH and LH, as it interferes with lipid synthesis.
The HepG2 cells were treated with palmitic acid and were treated with variety concentrations of water extracts of Agastachis Herba(AH) or Lysimachiae Herba(LH). The lipotoxicity was assessed using EZ-cytox and the lipoapoptosis was assessed using cell death detection ELISA. The intracellular lipid was measured by staining of oil red O. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c(SREBP-1c), fatty acid synthase(FAS) and acetyl-CoA carboxylase(ACC) in HepG2 cells was measured by RT-PCR.
AH and LH increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC and FAS (SREBP-1c, ACC and FAS are factors involved in lipid synthesis). In the experiment of staining oil red O, Both extracts reduced intracellular lipid accumulation and LH''s efficacy is superior to AH''s efficacy.
According to the results, AH and LH are likely to contribute to non-alcoholic fatty liver disease, both AH and LH, as it interferes with lipid synthesis.
목차
ABSTRACT ⅲⅠ. 서 론 1Ⅱ. 재료 및 방법 31. 재료 31) 약재 32. 방법 31) 세포 배양 32) 지방증 유발 33) cell viability assay 4 4) RT-PCR 45) Lipotocity 측정 56) Oil Red O staining 63. 통계처리 6Ⅲ. 결 과 71. AH가 cell viability에 미치는 영향 72. LH가 cell viability에 미치는 영향 93. AH가 lipotoxicity에 미치는 영향 114. LH가 lipotoxicity에 미치는 영향 135. AH가 lipoapoptosis에 미치는 영향 156. LH가 lipoapoptosis에 미치는 영향 177. AH가 세포 지방 축적에 미치는 영향 198. LH가 세포 지방 축적에 미치는 영향 209. AH가 SREBP-1c 발현에 미치는 영향 2110. LH가 SREBP-1c 발현에 미치는 영향 2311. AH가 ACC에 미치는 영향 2512. LH가 ACC에 미치는 영향 2713. AH가 FAS에 미치는 영향 2914. LH가 FAS에 미치는 영향 31Ⅳ. 고 찰 33Ⅴ. 결 론 37참고문헌 38
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