지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 발행연도
- 2018
- 저작권
- 동의대학교 논문은 저작권에 의해 보호받습니다.
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Cheongnoemyeongsin-hwan (CNMSH), a herb medicine to treat cognitive
impairment, has been reported to have anti-inflammatory and antioxidant
efficacy in several experimental models. However, whether CNMSH can
ameliorate the amyloid-β (Aβ)-mediated cognitive impairments and
neurodegeneration in AD is unknown. To evaluate the protective effects of
CNMSH against Aβ-induced neuronal damage in vivo, CNMSH was
administered to mice for 2 weeks. The effects of dietary supplementation of
CNMSH on memory and learning skills in the Aβ-induced cognitive
impaired mice were explored using three different paradigms: the Morris
water maze, the T-maze spontaneous alternation task and the novel object
recognition task. Administration of CNMSH significantly attenuated Aβ
-mediated impairment of cognition and memory. CNMSH also effectively
attenuated Aβ-induced apoptosis and DNA damage, and inhibited the
expression of inducible nitric oxide synthase and cyclooxygenase-2. At the
same time, CNMSH reversed Aβ-induced effect on major AD markers (i.e.,
tau phosphorylation and β-secretase expression) and improved brain-derived
neurotrophic factor levels, suggesting that CNMSH improves Aβ-induced
memory deficits and cognitive impairment through regulation of these
proteins expression. Therefore, CNMSH has the potential to be a disease
modifying agent for the prevention of AD pathogenesis.
impairment, has been reported to have anti-inflammatory and antioxidant
efficacy in several experimental models. However, whether CNMSH can
ameliorate the amyloid-β (Aβ)-mediated cognitive impairments and
neurodegeneration in AD is unknown. To evaluate the protective effects of
CNMSH against Aβ-induced neuronal damage in vivo, CNMSH was
administered to mice for 2 weeks. The effects of dietary supplementation of
CNMSH on memory and learning skills in the Aβ-induced cognitive
impaired mice were explored using three different paradigms: the Morris
water maze, the T-maze spontaneous alternation task and the novel object
recognition task. Administration of CNMSH significantly attenuated Aβ
-mediated impairment of cognition and memory. CNMSH also effectively
attenuated Aβ-induced apoptosis and DNA damage, and inhibited the
expression of inducible nitric oxide synthase and cyclooxygenase-2. At the
same time, CNMSH reversed Aβ-induced effect on major AD markers (i.e.,
tau phosphorylation and β-secretase expression) and improved brain-derived
neurotrophic factor levels, suggesting that CNMSH improves Aβ-induced
memory deficits and cognitive impairment through regulation of these
proteins expression. Therefore, CNMSH has the potential to be a disease
modifying agent for the prevention of AD pathogenesis.
목차
Ⅰ. 서 론 ······················································································································· 1Ⅱ. 재료 및 방법········································································································· 41. 실험동물 ················································································································· 42. 시료준비 ················································································································· 43. Aβ25-35에 의한 실험동물의 기억 손상 유발 ··················································· 54. 물체 인지 실험(Novel object recognition) ····················································· 85. T-미로(T-maze) 실험························································································ 86. 수중 미로 실험(Water maze test) ··································································· 87. 혈액학적 검사····································································································· 118. 혈액 생화학적 검사··························································································· 119. 뇌 조직의 분리 및 Western blot analysis에 의한 단백질 발현 조사·· 1110. Hematoxylin 및 Eosin Y (H&E) 염색······················································· 1211. Bcl-2의 면역조직화학염색············································································· 1312. 통계분석············································································································· 13Ⅲ. 결 과 ····················································································································· 141. 청뇌명신환이 물체 인지 능력에 끼치는 효과 ············································· 142. 청뇌명신환이 T-maze test를 통한 인지능력에 끼치는 효과 ·················· 163. 청뇌명신환이 Water maze test를 통한 기억력 개선에 끼치는 효과 ···· 184. Aβ25-35 유도 AD 마우스 뇌의 BDNF, Tau 및 BACE 단백질 발현에 끼치는 청뇌명신환의 영향··················································································· 205. Aβ25-35 유도 AD 마우스 뇌 세포의 DNA 손상과 세포 사멸에 끼치는청뇌명신환의 영향····························································································· 226. Aβ25-35 유도 AD 마우스 뇌 세포에서 염증성 인자 발현에 끼치는 청뇌명신환의 영향 ····································································································· 247. Aβ25-35 유도 AD 마우스 뇌 조직의 병리학적 현상에 끼치는 청뇌명신환의 영향················································································································· 268. Aβ25-35 주사 및 청뇌명신환 경구 투여가 체중 및 장기 무게에 끼치는영향······················································································································· 299. Aβ25-35 주사 및 청뇌명신환 경구 투여가 간 및 신장 기능에 끼치는 영향··························································································································· 3210. Aβ25-35 주사 및 청뇌명신환 경구 투여가 혈액 지표상 끼치는 영향··· 34Ⅳ. 고 찰 ···························································································································· 36Ⅴ. 결 론 ···························································································································· 42참고문헌 ····························································································································· 44Abstract ····························································································································· 48
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