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논문 기본 정보

자료유형
학위논문
저자정보

전용삼 (충남대학교, 忠南大學校 大學院)

지도교수
이긍주
발행연도
2018
저작권
충남대학교 논문은 저작권에 의해 보호받습니다.

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초록· 키워드

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Oxalis L. is recognized as a weed in Korea, but it can be used as a medicinal plant for hemorrhoids and burns in the private sector and also as a resource for ornamental plants. In foreign countries, various kinds of flowers and varieties are traded in overseas open market, and various types are commercially used because they are considered to have a horticultural value due to various colors of flower and leaf form of Oxalis. In Korea, however, there is confusion between each species due to the irrelevant distribution name. So far, it is recognized as a weed and it is not recognized as a flower material. Although, there was been a basic study on the genus Oxalis native to Korea, but there is still a lack of horticultural research. This study was carried out to collect basic data on morphological and genetic diversity for developing as a new horticultural material. The diameter and depth of the flower were measured using vernier calipers to measure the morphological diversity. Length and width of the flower and calyx were measured. In the case of flowers, the ratio of diameter to depth was calculated. For petals and calyx, the ratio of length to width was calculated. In addition, the length of one side of a pentagon (p = diameter × sin 36 ° or cos 54 °) inside the imaginary circle with the diameter of the flower as the diameter of the petal with respect to the width of the petal, reflecting the characteristic of the Oxalis flower having five actinomorphic petals was defined as P ratio. The colors were determined using the RHS color chart 5th edition (Royal Horticultural Society, UK) and were classified. Inflorescence and fragrance were also investigated. In order to examine genetic diversity, we extracted DNA, did PCR of ITS I and ITS II locus, and analyzed by base sequence analysis. Pairwise distance and Maximum Likelihood phylogeny tree was constructed. It was found that there were 9 groups in the diameter of flowers, 10 groups in the depth of flowers, 10 groups in the length of the petals, 10 groups in the width of the petals, except for the width of the calyx. 7 groups in length of calyx, 7 groups in flower diameter and depth ratio, 9 groups in ratio of petal length and width, 10 groups in ratio of length and width of calyx, and 8 groups in P ratio, so the morphological diversity of the form was found to be high. Especially, P ratio was found to be useful as an approximate measure of the morphology of the Oxalis. It can be applied to the varieties of other actinomorphic flowers, such as using the inscribed quadrilateral in case of four petals and the inscribed hexagon in case of six petals. The Oxalis flowers appeared to be 13 colors, which was divided into 7 diverse groups. Through the ITS locus, genetic diversity was found to be divided into 3 types of caulescent, rhizome with scales and bulb according to the underground type. However, we could not find a trait that showed the same tendency as the nucleotide sequence analysis in the bulbar Oxalis, which could be solved by exploring and analyzing other useful segments in future species identification. As a result of investigating the diversity of bulbar Oxalis, morphological and genetic diversity could be examined and this could be used as a basic data to breed new varieties suitable for consumers'' preference in future as a new horticultural material.

목차

목 차 i
List of Tables iii
List of Figures iv
1. 서 론 1
2. 재료 및 방법 3
2-1. 실험 재료 3
2-2. 꽃의 크기 측정 및 분석 3
2-3. 화색 조사 및 계열 분류 4
2-4. 사진 촬영 4
2-5. 화서 및 향기 조사 4
2-6. ITS I 및 ITS II 구간에 대한 유전 다양성 확인 7
2-6-1. DNA extraction 7
2-6-2. PCR (Polymerase Chain Reaction) 분석 8
2-6-3. Gel extraction 9
2-6-4. 염기서열 정렬 및 계통 분석 9
3. 결과 및 고찰 10
3-1. 꽃의 크기 측정 및 분석 10
3-1-1. 꽃의 직경(diameter) 10
3-1-2. 꽃의 깊이(depth) 10
3-1-3. 꽃잎의 길이(length) 10
3-1-4. 꽃잎의 너비(width) 11
3-1-5. 꽃받침의 길이(length) 11
3-1-6. 꽃받침의 너비(width) 11
3-1-7. 꽃의 직경(diameter)과 깊이(depth)의 비율 12
3-1-8. 꽃잎의 길이(length)와 너비(width)의 비율 12
3-1-9. 꽃받침의 길이(length)와 너비(width)의 비율 13
3-1-10. P ratio 13
3-2. 화색 조사 및 계열 분류 18
3-3. 화서 및 향기 18
3-4. ITS I 및 ITS II 구간에 대한 유전 다양성 확인 22
3-4-1. 염기서열 분석 결과 22
3-4-2. ITS I 22
3-4-3. ITS II 23
3-4-4. ITS I 및 ITS II 24
4. 결 론 35
5. 참고문헌 37
ABSTRACT 40

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