지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김순중
- 발행연도
- 2018
- 저작권
- 세명대학교 논문은 저작권에 의해 보호받습니다.
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Objectives
This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2.
Methods
Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA).
Results
The GT does not impair the cell viability in tested concentration 500μg/ml or below. GT significantly reduced the NO production in a dose-dependent manner. GT 500μg/ml also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT 500μg/ml reduced the PGE2 secretion in LPS induced RAW264.7 cells.
Conclusions
These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT''s anti-inflammatory effect objectively.
This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2.
Methods
Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA).
Results
The GT does not impair the cell viability in tested concentration 500μg/ml or below. GT significantly reduced the NO production in a dose-dependent manner. GT 500μg/ml also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT 500μg/ml reduced the PGE2 secretion in LPS induced RAW264.7 cells.
Conclusions
These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT''s anti-inflammatory effect objectively.
목차
Ⅰ. 서 론 1Ⅱ. 재료 및 방법 11. 재료 11) 약재 32) Raw264.7 세포 33) 시약 52. 방법 61) 세포배양 62) Raw264.7 세포 독성 시험 63) NO 생성 억제효과 측정 74) RNA 추출 75) iNOS, COX-2의 mRNA 발현 측정 86) Prostaglandin E2 (PGE2) 측정 97) 통계 분석 10Ⅲ. 결 과 111. 세포 독성 측정 112. NO 측정 123. iNOS 발현 결과 144. COX-2 발현 결과 165. PGE2 생성 억제 결과 18Ⅳ. 고 찰 20Ⅴ. 결 론 25참 고 문 헌 27Abstract 32
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