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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 김윤식
- 발행연도
- 2018
- 저작권
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The purpose of this study was to investigate the effect of Fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salvia miltiorrhiza Bunge(FMH) on Anti-inflammation associated with Dyslipidemia and Anti-oxidation in RAW264.7 and HUVEC Cell.
at first, total polyphenol, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity and safety in cytotoxicity of FMH were measured. after treatment with FMH in RAW264.7 cell, production of NO, cytokines, LTB4 were measured and after treatment with FMH in HUVEC cell, production of cDNA of genes related to inflammation were measured.
The result were summarized as follows :
1. FMH contained polyphenol and flavonoids. and DPPH and ABTS radical scavenging activity increased in FMH in a concentration-dependent manner.
2. FMH showed inhibitory effect on nitric oxide(NO), cytokines and LTB4 in RAW264.7 cell compared to control group.
3. FMH decreased transcription of pro-inflammatory genes, whereas increased transcription of anti-inflammatory genes in HUVEC cell.
These results suggest that FMH is effective in antioxidation and treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke and other cardiocerebrovascular disease.
at first, total polyphenol, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity and safety in cytotoxicity of FMH were measured. after treatment with FMH in RAW264.7 cell, production of NO, cytokines, LTB4 were measured and after treatment with FMH in HUVEC cell, production of cDNA of genes related to inflammation were measured.
The result were summarized as follows :
1. FMH contained polyphenol and flavonoids. and DPPH and ABTS radical scavenging activity increased in FMH in a concentration-dependent manner.
2. FMH showed inhibitory effect on nitric oxide(NO), cytokines and LTB4 in RAW264.7 cell compared to control group.
3. FMH decreased transcription of pro-inflammatory genes, whereas increased transcription of anti-inflammatory genes in HUVEC cell.
These results suggest that FMH is effective in antioxidation and treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke and other cardiocerebrovascular disease.
목차
목 차 ⅰ표 목 차 ⅳ그림목차 ⅴⅠ. 서 론 1Ⅱ. 재료 및 방법 31. 재료 31) 약재 32) 시약 43) 기기 52. 방법 61) 시료 추출 62) 항산화 효능평가 7(1) 총 polyphenol 함량 측정 7(2) 총 flavonoid 함량 측정 7(3) DPPH radical 소거능 측정 7(4) ABTS radical 소거능 측정 83) 세포 배양 94) 세포 생존율 측정 95) 항염증 효능평가 9(1) 세포 내 nitric oxide (NO) 생성량 측정 9(2) 세포 내 cytokine 생성량 측정 10(3) 세포 내 LTB4 생성량 측정 106) 세포 내 유전자 발현량 측정 11(1) RNA 추출 11(2) cDNA 합성 12(3) 유전자 발현량 측정 133. 통계처리 15Ⅲ. 실험 결과 161. 항산화 효능평가 161) 총 polyphenol 함량 측정 162) 총 flavonoids 함량 측정 173) DPPH radical 소거능 측정 184) ABTS radical 소거능 측정 192. 세포 생존율 측정 203. 항염증 효능평가 231) 세포 내 NO 생성량 측정 232) 세포 내 cytokine 생성량 측정 24(1) IL-1β 24(2) IL-6 25(3) TNF-α 263) 세포 내 LTB4 생성량 측정 274. 세포 내 유전자 발현량 측정 281) CCL5 282) MCP-1 293) CXCL8 304) CX3CL1 315) ICAM-1 326) VCAM-1 337) KLF2 348) eNOS 35Ⅳ. 고 찰 36Ⅴ. 결 론 45참 고 문 헌 46영 문 초 록 54
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