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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학위논문
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- 지도교수
- 윤대환
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- 2019
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Abstract
The effects of Acupuncture at LR3 acupoint on Mitochondrial Respiratory Chain activity in Rat Liver
Dong-Hee Choi
Dept. of Korean Medicine
Graduate School of Dongshin University
(Directed by Prof. Dae-Hwan Youn, O.M.D., Ph.D.)
Objective : There are many mitochondria in the liver, and mitochondria are intracellular double membrane-bound structures that supply energy for intracellular metabolism includes Krebs cycle and beta-oxidation. Also, acupuncture has been known to stimulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activity in rat liver, the activity of mitochondrial respiratory chain complexes I to IV were observed.
Methods : The rats were divided into four groups; Normal1(no acupuncture treatment and under anesthesia for 5 min), Normal2(no acupuncture treatment under anesthesia for 10 min), MA1(acupuncture treatment at LR3 acupoint under anesthesia for 5 min), MA2(acupuncture treatment at LR3 acupoint under anesthesia for 10 min). All rats were sacrificed and the livers were examined for respiratory chain change.
Results : There was no differenced in Ubiquinon oxidoreductase, Succinate dehydrogenase, Ubiquinol Cytochrome C Oxidoreductaseon on acupuncture at LR3 acupoint. Acupuncture at LR3 Acupoint increased activity of Cytochrome C oxidase.
Conclusion : Acupuncture at LR3 acupoint mediated mitochondrial repiratory chain activity via the Cytochrome C oxidase signaling pathway in liver of rats.
Keywords : LR3 Acupoint, Ubiquinon oxidoreductase, Succinate dehydrogenase, Ubiquinol Cytochrome C Oxidoreductase, Cytochrome C Oxidase
The effects of Acupuncture at LR3 acupoint on Mitochondrial Respiratory Chain activity in Rat Liver
Dong-Hee Choi
Dept. of Korean Medicine
Graduate School of Dongshin University
(Directed by Prof. Dae-Hwan Youn, O.M.D., Ph.D.)
Objective : There are many mitochondria in the liver, and mitochondria are intracellular double membrane-bound structures that supply energy for intracellular metabolism includes Krebs cycle and beta-oxidation. Also, acupuncture has been known to stimulate the flow of energy. To explore the effect of acupuncture on the mitochondrial respiratory chain activity in rat liver, the activity of mitochondrial respiratory chain complexes I to IV were observed.
Methods : The rats were divided into four groups; Normal1(no acupuncture treatment and under anesthesia for 5 min), Normal2(no acupuncture treatment under anesthesia for 10 min), MA1(acupuncture treatment at LR3 acupoint under anesthesia for 5 min), MA2(acupuncture treatment at LR3 acupoint under anesthesia for 10 min). All rats were sacrificed and the livers were examined for respiratory chain change.
Results : There was no differenced in Ubiquinon oxidoreductase, Succinate dehydrogenase, Ubiquinol Cytochrome C Oxidoreductaseon on acupuncture at LR3 acupoint. Acupuncture at LR3 Acupoint increased activity of Cytochrome C oxidase.
Conclusion : Acupuncture at LR3 acupoint mediated mitochondrial repiratory chain activity via the Cytochrome C oxidase signaling pathway in liver of rats.
Keywords : LR3 Acupoint, Ubiquinon oxidoreductase, Succinate dehydrogenase, Ubiquinol Cytochrome C Oxidoreductase, Cytochrome C Oxidase
목차
목 차ABSTRACT ⅲI. 서론 1II. 재료 및 방법 31. 실험 재료 31) 실험동물 32) 침자 32. 실험 방법 31) 군분리 및 시술 32) 취혈 및 침자 43) 채혈 및 Plasma 분리 44) 조직준비 45) Mitochondria 분리 및 측정 56) Ubiquinon Oxidoreductase(complexⅠ)측정 57) Succinate Dehydrogenase(complex Ⅱ)측정 68) Ubiquinol Cytochrome C Oxidoreductase(Complex III)측정 79) Cytochrome C oxidase(complex Ⅳ)측정 73. 통계 처리 8III. 실험 결과 91. Liver to body weight 변화 92. Mitochondrial protein concentration 변화 103. Ubiquinon Oxidoreductase (complexⅠ) 변화 114. Succinate Dehydrogenase (complex Ⅱ) 변화 125. Ubiquinol Cytochrome C Oxidoreductase (Complex III) 변화 136. Cytochrome C oxidase (complex Ⅳ) 변화 147. Aspartate aminotransferase(AST) 변화 158. Alanine aminotransferase(ALT) 변화 169. Total protein 변화 17IV. 고 찰 18V. 결 론 24참고문헌 26
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