This study was carried out by collecting soil from Jeonnam province using a soil test method. The purpose was to investigate the occurrence patterns of paddy weeds, their resistance levels to an ALS inhibiting herbicide, and to estimate the areas of resistance in these paddy fields. Additionally, Echnochloa oryzoides, E. oryzoides (Ard.) Fritsch and E. crus-galli var. echinata accessions collected from Jeonnam province were tested in order to determine their resistance levels to ACCase and ALS inhibiting herbicides. I used rapid diagnosis (growth pouch) and whole plant tests. I used soil collected from 358 paddy fields of Jeonnam province in 2017. Based on their life cycles, weeds were 96% annuals and 4% perennial. Additionally, according to morphological classification, 59% were broad leaves, 28% were sedges and 13% were grasses. Different areas within Jeonnam province contained different numbers and occurrence rates of weed species. However, generally, we observed Lindernia dubia var. dubia, Monochoria vaginalis var. plantaginea, Ludwigia prostrata, Lindernia procumbens, Cyperus difformis, Scirpus juncoides, Eleocharis Kuroguwai, Echinochloa oryzoides, and E. crus-galli var. echinata. I also observed seven weeds resistant to an ALS inhibiting herbicide. They were M. vaginalis, S. juncoides, C. difformis, L. dubia, Ludwigia prostrata, E. oryzoides, and E. crus-galli var. echinata. Although there were differences in the number and occurrence rate of resistant weed species to an ALS inhibiting herbicide among areas in Jeonnam province, the M. vaginalis, C. difformis, and S. juncoides occurred in 23 cities and counties in Jeonnam including Gwangju metropolitan city. Based on the rates (52%) of resistant occurrence to an ALS inhibiting herbicide in Jeonnam province, the area of weed resistant paddy fields was estimated to be 91,543 ha. In a rapid diagnois test of the accessions, 63% of E. oryzoides, 43% of E. oryzoides (Ard.) Fritsch and 25% of E. crus-galli var. echinata were resistant to the ALS inhibiting herbicide penoxsulam. Addtionally, 73% of E. oryzoides, 34% of E. oryzoides (Ard.) Fritsch and 60% of E. crus-galli var. echinata were resistant to the ACCase inhibiting herbicide cyhalofop-butyl. These accessions were all collected from Jeonnam province. I then conducted whole plant test. I found that in both the rapid diagnosis and whole plant tests, the plants'' resistances levels to the ALS inhibiting herbicide penoxsulam were often the same, regardless of which test was used. For example, in E. oryzoides the resistant levels were the same 83% of the time. In E. oryzoides (Ard.) Fritsch, they were the same 74% of the time and in E. crus-galli var. echinata they were the same 54% of the time. When I compared rapid diagnosis and whole plant tests'' measurements of resistance to the ACCase inhibiting herbicide cyhalofop-butyl, I found that the resistance levels were the same, regardless of which test was used, 67% of the time in E. oryzoides, 83% of the time in E. oryzoides (Ard.) Fritsch and 75% of the time in E. crus-galli var. echinata. Therefore, the resistance of paddy weeds to ALS and ACCase inhibiting herbicides can effectively be detected using a rapid diagnosis method such as a growth pouch.
목차
Ⅰ. 서 언 1Ⅱ. 연 구 사 3Ⅲ. 재료 및 방법 51. 토양검정법 52. Growth pouch test를 이용한 전남지역 피에 대한 penoxsulam과 cyhalofop-butyl 저항성 검정 73. Whole plant test를 이용한 전남지역 피에 대한 penoxsulam과 cyhalofop-butyl 저항성 검정 8Ⅳ. 결과 및 고찰 91. 토양검정법 92. Growth pouch test를 이용한 전남지역 피에 대한 penoxsulam과 cyhalofop-butyl 저항성 검정 223. Whole plant test를 이용한 전남지역 피에 대한 penoxsulam과 cyhalofop-butyl 저항성 검정 374. Growth pouch test와 whole plant test 저항성 검정 비교 50Ⅴ. 요 약 62Ⅵ. 인 용 문 헌 64Ⅶ. 부 록 72