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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 곽효범
- 발행연도
- 2019
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- 인하대학교 논문은 저작권에 의해 보호받습니다.
이용수2
이 논문의 연구 히스토리 (2)
2019
Effects of A Single Bout Exercise on Mitochondrial Function, Dynamics, Mitophagy, and Mitochondria-Mediated Apoptotic Signaling in Rat Cardiac and Skeletal Muscles : A Single Bout Exercise and Mitochondria in Cardiac and Skeletal Muscles
유수지
체육학과
2019.01
학위논문
Effects of Acute Exercise on Mitochondrial Function, Dynamics, and Mitophagy in Rat Cardiac and Skeletal Muscles
유수지
,
노미현
,
허준원
외 7명
International Neurourology Journal
2019.01
학술저널
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초록· 키워드
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The current study aimed to determine the effects of a single bout exercise on mitochondrial function, dynamics (fusion and fission), mitophagy, and mitochondria-mediated apoptotic signaling in cardiac and skeletal muscles. Fischer 344 rats (4 months old) were randomly divided into the control (CON) or a single bout exercise (EX) group (n = 10 each). The rats performed a single bout treadmill exercise for 60 minutes. Mitochondrial function (e.g., O2 respiration, H2O2 emission, and Ca2+ retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, and Opa1), mitochondrial fission (e.g., Drp1 and Fis1), mitophagy (e.g., Parkin, Pink1, LC3II, and Bnip3), and mitochondria-mediated apoptotic signaling (e.g., Bax, Bcl-2, mPTP opening, cytochrome c, and cleaved caspase-3) were measured in cardiac (e.g., left ventricle) and skeletal (e.g., soleus and white gastrocnemius) muscles. Mitochondrial O2 respiration and Ca2+ retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial H2O2 emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after a single bout exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences; It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle. A single bout exercise significantly decreased mPTP opening sensitivity in all tissues. However, a single bout exercise didn’t show any statistical differences of Bax, Bcl-2, cytochrome c, and cleaved caspase-3 in all tissues. A single bout exercise may improve mitochondrial function (e.g., O2 respiration and Ca2+ retention capacity) in the cardiac and skeletal muscles without changes in mitochondrial dynamics, mitophagy, and mitochondria-mediated apoptotic signaling.
목차
Table of ContentsAbstract ................................................................................................................................ iList of Figures .................................................................................................................. ivI. Introduction1. Significance .......................................................................................................... 12. Purpose ................................................................................................................. 33. Hypotheses ........................................................................................................... 3II. Experiment Ⅰ: Effects of a single bout exercise on mitochondrial function, dynamics, and mitophagy in rat cardiac and skeletal muscles1. Introduction............................................................................................................ 42. Materials and methods ......................................................................................... 61) Animals and ethical approval .......................................................................... 62) Exercise design ................................................................................................ 63) Mitochondrial function .................................................................................... 64) Western immunoblot analysis .......................................................................... 75) Statistical analysis ........................................................................................... 83. Results ................................................................................................................. 91) Effects of a single bout exercise on mitochondrial function in cardiacand skeletal muscles ......................................................................................... 92) Effects of a single bout exercise on mitochondrial dynamics(fusion and fission) and mitophagy in cardiac and skeletal muscles .............. 114. Discussion .......................................................................................................... 15III. Experiment Ⅱ: Effects of a single bout exercise on apoptosis in rat cardiac and skeletal muscles1. Introduction.......................................................................................................... 192. Materials and methods ....................................................................................... 211) Experimental design ...................................................................................... 212) Animals and ethical approval ........................................................................ 213) Exercise design .............................................................................................. 214) Western immunoblot analysis ........................................................................ 215) Statistical analysis ......................................................................................... 223. Results ............................................................................................................... 231) Effects of a single bout exercise on Bax and Bcl-2 in cardiac andskeletal muscles ............................................................................................. 232) Effects of a single bout exercise on mPTP, cytochrome c, andcleaved caspase-3 in cardiac and skeletal muscles ........................................ 244. Discussion .......................................................................................................... 25IV. Conclusion ...................................................................................................... 27References ............................................................................................................... 28
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