목차

CONTENTS
INTRODUCTION 1
MATERIALS & METHODS 5
1. Animals and experimental design 5
2. Histopathology 6
3. Immunohistochemistry 6
4. Immunoblot analysis 7
5. Serum biochemistry for the measurement of ALT and Vitamin C level 8
6. Liver triglyceride measurement 9
7. Quantitative RT-PCR 9
8. Cell culture and in vitro experiments 11
9. Statistical analysis 12
RESULTS 13
1. There was no difference in the values of diet ingestion / body weight between WT mice and SMP30 KO mice 13
2. SMP30 depletion exhibited significantly lower liver weight compared to that of high-fat diet fed WT mice 15
3. Hepatocyte sizes of SMP30 KO mice were significantly smaller compared with those of WT mice. 18
4 SMP30 KO mice showed significantly lower values of histopathological liver steatosis grade compared with WT mice. 21
5. SMP30 depletion exhibited much less triglyceride accumulation in the liver compared to high-fat diet fed WT mice 25
6. There was no significant change in SMP30 expression level among all WT groups. 28
7. SMP30 KO mice revealed significantly reduced serum vitamin C and ALT levels compared with WT mice 30
8. Serum triglyceride level was lower in SMP30 KO mice compared with WT mice 32
9. Enhanced levels of β-oxidation and cellular fatty acid uptake related mRNA are associated with depletion of SMP30 in the liver of high-fat diet fed mice 34
10. SMP30 KO mice also exhibited significantly higher levels of lipogenic mRNA compared with those of WT mice 38
11. Activation of AMPK (AMP activated protein kinase) was dramatically enhanced by SMP30 depletion in the liver of high-fat diet fed mice 40
12. Vitamin C supplements significantly increased intracellular triglyceride level in oleic acid treated AML 12 hepatocytes 42
DISCUSSION 44
ABSTRACT 53
REFERENCES 55
국문초록 60