지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 李孝淵
- 발행연도
- 2020
- 저작권
- 제주대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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H i b i s c u s s y r i a c u s L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus ‘Nanpa’ using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA
and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus ‘Nanpa’ can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus ‘Nanpa’ in the future
and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot-forming callus production. Our findings show that mature leaves of H. syriacus ‘Nanpa’ can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus ‘Nanpa’ in the future
목차
요약 4표 차례 5그림 차례 6약어 7I. 서론 8II. 재료 및 방법 121. 실험재료 및 소독 122. 배지 조건 133. 캘러스유도 144. 식물체의 재분화 15III. 결과 및 고찰 191. 잎 절편으로부터 캘러스 유도 192. 재분화에 영향을 주는 캘러스 조건 203. 재분화 과정에서의 형태발생과 체세포 변이관찰 21Ⅳ 종합고찰 33Ⅴ. 참고문헌 34
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