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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 설인찬
- 발행연도
- 2020
- 저작권
- 대전대학교 논문은 저작권에 의해 보호받습니다.
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초록· 키워드
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The purpose of this study was to investigate the antioxidant, anti-inflammatory and anti-cellular adhesion molecules effects of Allii Macrostemonis Bulbus, Artemisiae Capillaris Herba, Crataegi Fructus, Curcumae Radix, Salviae Militiorrhizae Radix complex extract(AMCP) in HUVEC.
We measured DPPH radical scavenging activity and ABTS radical scavenging activity of AMCP to evaluate its antioxidant effect. and we also measured the expression level of NF-κB, IκBα, ERK, JNK, p38 proteins to evaluate its anti-inflammatory effect. Lastly, we measured the expression level of MCP-1, ICAM-1, VCAM-1 mRNA and their level to evaluate its anti-celluar adhesion molecules.
Results were summarized as follows :
1. AMCP did not show any cytotoxicity in HUVEC within the concentraion tested except for a concentration of 400 ㎍/㎖.
2. AMCP increased the DPPH radical scavenging activitiy and ABTS radical scavenging activity in HUVEC as the concentration of AMCP rises.
3. AMCP significantly reduced NF-κB, IκBα, JNK, and p38 protein expression in HUVEC at concentrations of 100, 200 μg/ml compared to control group, and significantly reduced ERK protein expression in HUVEC at all concentrations compared to control group.
4. AMCP significantly reduced MCP-1 gene expresion in HUVEC at 200 μg/ml compared to control group, and significantly reduced ICAM-1 and VCAM-1 gene expression in HUVEC at 100, 200 μg/ml compared to control group.
5. AMCP significantly decreased the levels of MCP-1, ICAM-1, VCAM-1 in HUVEC at concentrations of 100, 200 μg/ml compared to control group.
These results suggest that AMCP has effect on antioxidation, anti-inflammation and anti-celluar adhesion molecule, which help the treatment and prevention of dyslipidemia and atherosclerosis.
We measured DPPH radical scavenging activity and ABTS radical scavenging activity of AMCP to evaluate its antioxidant effect. and we also measured the expression level of NF-κB, IκBα, ERK, JNK, p38 proteins to evaluate its anti-inflammatory effect. Lastly, we measured the expression level of MCP-1, ICAM-1, VCAM-1 mRNA and their level to evaluate its anti-celluar adhesion molecules.
Results were summarized as follows :
1. AMCP did not show any cytotoxicity in HUVEC within the concentraion tested except for a concentration of 400 ㎍/㎖.
2. AMCP increased the DPPH radical scavenging activitiy and ABTS radical scavenging activity in HUVEC as the concentration of AMCP rises.
3. AMCP significantly reduced NF-κB, IκBα, JNK, and p38 protein expression in HUVEC at concentrations of 100, 200 μg/ml compared to control group, and significantly reduced ERK protein expression in HUVEC at all concentrations compared to control group.
4. AMCP significantly reduced MCP-1 gene expresion in HUVEC at 200 μg/ml compared to control group, and significantly reduced ICAM-1 and VCAM-1 gene expression in HUVEC at 100, 200 μg/ml compared to control group.
5. AMCP significantly decreased the levels of MCP-1, ICAM-1, VCAM-1 in HUVEC at concentrations of 100, 200 μg/ml compared to control group.
These results suggest that AMCP has effect on antioxidation, anti-inflammation and anti-celluar adhesion molecule, which help the treatment and prevention of dyslipidemia and atherosclerosis.
목차
Ⅰ. 서 론 1Ⅱ. 연구 방법 31. 재료 31) 시료 32) 시약 43) 기기 52. 방법 61) 시료 추출 62) 세포 배양 63) 세포생존율 측정 64) DPPH radical 소거능 측정 75) ABTS radical 소거능 측정 76) 단백질 발현량 측정 87) 유전자 발현량 측정 9(1) RNA 추출 9(2) cDNA 합성 9(3) 유전자 증폭 108) 바이오마커 생성량 측정 113. 통계처리 12Ⅲ. 연구 결과 131. 세포생존율 132. DPPH radical 소거능 153. ABTS radical 소거능 164. 단백질 발현량 171) NF-κB 172) IκBα 193) ERK 204) JNK 215) p38 225. 유전자 발현량 231) MCP-1 232) ICAM-1 253) VCAM-1 266. 바이오마커 생성량 271) MCP-1 272) ICAM-1 293) VCAM-1 30Ⅳ. 고 찰 31Ⅴ. 결 론 37참 고 문 헌 38영 문 초 록 50
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