목차

Contents
1. Introduction ····························································· 1
1.1 Neural circuit: in vivo and in vitro ······························· 1
1.2 MEMS neural probe for investigating neural circuits ········· 2
1.3 Method for modulating neural circuits ·························· 4
1.4 Multifunctional neural probe ····································· 5
1.5 Approach for investigating and modulating neural circuits ·· 7
2. Multifunctional multi shank neural probe for investigating and modulating long-range neural circuit in vivo··············· 9
2.1 Summary ···························································· 9
2.2 Fabrication and characterizations of multifunctional multi-shank neural probe ················································· 10
2.2.1 Design of multifunctional multi-shank neural probe ··· 10
2.2.2 Fabrication and packaging of multifunctional multi-shank neural probe ················································· 11
2.2.3 Characterizations of multifunctional multi-shank neural probe ························································· 16
2.3 In vivo demonstration of multifunctional neural probe ······· 20
2.3.1 Immune responses by the multifunctional probe in the brain ································································· 20
2.3.2 Localized optical stimulation with multifunctional probe array ·························································· 25
2.4 Investigation and modulation of hippocampal CA3 and CA1 circuit ································································ 28
2.4.1 Investigation of hippocampal CA3 and CA1 connectivity ························································· 28
2.4.2 Modulation of hippocampal CA3 and CA1 connectivity ························································· 32
2.5 Discussion and conclusion ········································ 36
3. 3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics in vitro······················································· 38
3.1 Summary ···························································· 38
3.2 Fabrication and characterizations of the 3D multifunctional MEA ································································· 39
3.2.1 Fabrication of the 3D multifunctional MEA ············ 39
3.2.2 Packaging of the 3D multifunctional MEA ·············· 42
3.2.3 Characterizations of the 3D multifunctional MEA ····· 44
3.2.4 The system assembly ······································· 47
3.3 3D neural culture on the 3D multifunctional MEA system ·· 48
3.3.1 Culture method of the single-group and two-group 3D neural network models ············································ 48
3.3.2 The single-group and two-group 3D neural network models ······························································· 51
3.4 Investigation of neural circuit dynamics in the single-group and two-group 3D neural network models ····················· 53
3.4.1 Neural network changes in the single-group 3D neural network model ······················································ 53
3.4.2 Analysis of neural circuit dynamics by local stimulation in the single-group 3D neural network model ··· 59
3.4.3 Functional connectivity between somatic regions in the two-group 3D neural network model ······················· 65
3.5 Discussion and conclusion ········································ 72
4. Wireless neural probe system for investigating neural circuits in freely behaving mice······································ 74
4.1 Summary ···························································· 74
4.2 Design and fabrication of wireless neural probe system ······ 75
4.2.1 Design of wireless neural recording system ············· 75
4.2.2 Packaging of wireless neural probe system ·············· 78
4.3 In vivo demonstration of wireless neural probe system ······ 78
4.3.1 Recording performance of wireless neural probe system ······························································· 78
4.3.2 Recording distance and long-term recording of wireless neural probe system ····································· 81
4.3.3 Open-field test ··············································· 82
4.3.4 Simultaneous recording from the multiple mice ········ 84
4.4 Discussion and conclusion ········································ 86
5. Conclusion ······························································· 88
5.1 Summary ···························································· 88
5.2 Outlook ······························································ 90
Reference ································································· 92