Grape (Vitis spp, 2n = 38) is consumed as wine, raisins and fresh fruits the interest in the development of new grape varieties is still increasing. However, visual selection during grape breeding is time consuming and labor intensive. In the past decades, many studies were reported on the development of grape genetic linkage maps and the identification of quantitative trait loci (QTL) for various fruit traits such as seedlessness, berry size, fruit ripening, fruit quality, and cluster structure. In this study, we aimed to identify QTLs for fruit traits through genotyping–by–sequencing (GBS) analysis and SNP marker development. A total of 192 F1 individuals derived from the cross between ''Tano Red'' (V. labrusca × V. vinifera) and ''Ruby Seedless'' (V. vinifera) was used for the QTL analysis and single nucleotide polymorphism (SNP) marker development. In the previous study, a total of 1,336 SNPs was used for designing primer sets for high–resolution melting (HRM) analysis. HRM analysis revealed the diallelic segregation in 805 of primer sets. In results, a total of 2,364 markers consisting of 1,929 GBS–based SNPs and 435 HRM markers was mapped on grape genetic linkage map. QTL analysis was performed on fruit traits such as fruit weight, fruit length, fruit width, length/width ratio, acidity, hardness flesh, hardness skin, seed number, seed weight, soluble solid, tensile strength, fruit skin PTC and fruit seed PTC using data over 1–4 years. As a result of QTL, among a total 58 QTLs, the one with the highest LOD value was selected. The major QTLs of fruit width, weight, length and seed weight was detected on chromosome 18.Two major QTLs associated with fruit skin PTC and seed PTC of fruit was identified on chromosome 11. Six major QTLs controlling flowering rate was identified on chromosome 5. QTL controlling acidity was detected on chromosome 2. Two QTLs tensile strength was detected on chromosome 18. Three QTLs associated with hardness flesh of fruit was identified on chromosome 11. QTL controlling hardness skin was detected on chromosome 11. QTL associated with soluble solid was detected on chromosome 16. QTL controlling seed number was detected on chromosome 18. Three QTLs associated with fruit length/width was detected on chromosome 18. This QTL information and the QTL–linked HRM markers are expected to be helpful for grape breeding.