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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2001.3
- 수록면
- 39 - 50 (12page)
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In order to investigate the effect of Transforming growth factor β1(below TGF-β1) and osteogenic protein-1(below OP-1) onto the myogenic differentiation, C2C12 satellite myoblastic cell line was cultured and treated with both growth factors. At first morphological changes with microscopical examination were examined, and isolated total RNA to analyse mRNA expression of bone marker proteins, muscle regulatory proteins, TGF-β receptor and their ligands by Northern blot analysis. And cellular proliferative inducibility of both growth factors was also tested to C2C12 cells.
Incubating the cell with 5 ng/㎖ of TGF-β1 until 4 days almost inhibited multinucleated myotube formation expressing muscular regulatory proteins, and induced decreasing Id proteins. However, no osteoblastic phenotypes was induced by TGF-β1 in C2C12 cells. The mRNA expression of TGF-β receptors with TGF-β1 was conversed after 48 hours cultured. Type I TGF-β receptor was seemed to play a role in negative signalling for inhibition of myogenic differentiation. OP-1 dose dependently induced ALP activity, osteopontine production and bone sialoprotein production at concentrations above 100ng/㎖ and osteocalcin production at concentrations above 300ng/㎖. The concentration of OP-1 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubation with above 100ng/㎖ OP-1 suppressed the expression of mRNA for muscular regulatory proteins from 2 days after incubation. Expression of Id-1, 2, 3 mRNA were stimulated by OP-1 at concentration above 300ng/㎖. When C2C12 cells were treated with both growth factors, TGF-β1 potentiated the inhibitory effect of OP-1 on myotube formation and expression of mRNA for myogenin at 12 days. And TGF-β1 reduced osteocalcin and bone sialoprotein production induced by OP-1 at 12 days in C2C12 cells. Both growth factor had no mitogenic effect. These results indicate that OP-1 converts the differentiation pathway of C2C12 myoblasts into that of osteoblastic lineage cells and it's not heritable, but TGF-β1 does not and has reversible inhibitory activity on the myogenic differentiation. TGF-β1 and OP-1 play a role in myogenic differentiation via different mechanism between them.
Incubating the cell with 5 ng/㎖ of TGF-β1 until 4 days almost inhibited multinucleated myotube formation expressing muscular regulatory proteins, and induced decreasing Id proteins. However, no osteoblastic phenotypes was induced by TGF-β1 in C2C12 cells. The mRNA expression of TGF-β receptors with TGF-β1 was conversed after 48 hours cultured. Type I TGF-β receptor was seemed to play a role in negative signalling for inhibition of myogenic differentiation. OP-1 dose dependently induced ALP activity, osteopontine production and bone sialoprotein production at concentrations above 100ng/㎖ and osteocalcin production at concentrations above 300ng/㎖. The concentration of OP-1 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubation with above 100ng/㎖ OP-1 suppressed the expression of mRNA for muscular regulatory proteins from 2 days after incubation. Expression of Id-1, 2, 3 mRNA were stimulated by OP-1 at concentration above 300ng/㎖. When C2C12 cells were treated with both growth factors, TGF-β1 potentiated the inhibitory effect of OP-1 on myotube formation and expression of mRNA for myogenin at 12 days. And TGF-β1 reduced osteocalcin and bone sialoprotein production induced by OP-1 at 12 days in C2C12 cells. Both growth factor had no mitogenic effect. These results indicate that OP-1 converts the differentiation pathway of C2C12 myoblasts into that of osteoblastic lineage cells and it's not heritable, but TGF-β1 does not and has reversible inhibitory activity on the myogenic differentiation. TGF-β1 and OP-1 play a role in myogenic differentiation via different mechanism between them.
목차
Ⅰ. 서 론
Ⅱ. 연구재료 및 연구방법
Ⅲ. 연구결과
Ⅳ. 고 찰
Ⅴ. 결 론
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