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자료유형
학술저널
저자정보
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한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.5 No.3
발행연도
2000.9
수록면
131 - 135 (5page)

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The inhibitory effect of various organic acids on the growth and survival of Escherichia coli O157:H7 in tryptic soy broth with 0.6% yeast extract at 37℃ or 4℃ was determined. Minimal inhibitory pHs of acetic acid, citric acid, fumaric acid, hydrochloric acid and lactic acid were 5.0, 4.0, 4.5, 4.0 and 4.5, respectively. Acetic acid (0.012 M) showed the strongest antimicrobial activity, based on the pH values or equivalent molar concentrations, followed by lactic acid (0.006 M), fumaric acid (0.004 M) and citric acid (0.004 M), respectively. E. coli O157:H7 with an initial in℃ulum of 10^7 CFU/ml and 10^5 CFU/ml in tryptic soy broth supplemented with 0.6% yeast extract, acidified to target pH with citric, fumaric and lactic acids at 37℃, was completely inactivated after 7 d and 5 d incubation, respectively, except for the acetic acid (9 d). The bactericidal effect decreased at the same pH when the incubation temperature was reduced from 37℃ to 4℃. The pH values of 0.2% acetic (pH 5.1), 0.6% citric (pH 4.2) and 0.4% lactic acid (pH 4.3) in TSBYE were almost correspondent to the minimal inhibitory pH values on E. coli O157:H7 of acetic (pH 5.0), citric (pH 4.0) and lactic acids (pH 4.5).

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Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

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