지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Epoxide hydrolase activities of various microbial cells were analyzed by colorimetric assay based on alkylation of epoxides with 4-(p-nitrobenzyl)pyridine (NBP). The epoxide hydrolase activity was determined by measuring the decrease of color intensity at 560 nm due to the decrease of styrene oxide substrate by epoxide hydrolase-catalyzed hydrolysis reaction. The experimental conditions of NBP colorimetric assay were optimized for the efficient measurement of epoxide hydrolase activities from various microbial cells.
목차
Abstract
실험재료 및 방법
결과 및 고찰
요약
참고문헌
참고문헌 (11)
참고문헌 신청
Archelas, A. and R. Furstoss. 2001. Synthetic applications of epoxide hydrolases. Current Opinion in Chem. Biol. 5, 112-119.
Doderer. K., S. Lutz-Wahl, B. Hauer and R. D. Schmid. 2003. Spectrophotometric assay for epoxide hydrolase activity toward any epoxide. Anal. Biochem. 321, 131-134.
Kasai, N., T. Suzuki, Y. Furukawa. 1998. Chiral C3 epoxides and halohydrins: their preparation and synthetic application. J. Mol. Cat. B: Enz. 4, 237-252.
Lee, E. Y., S.-S. Yoo, H. S. Kim, S. J. Lee, Y.-K. Oh and S. Park. 2004. Production of (S)-styrene oxide by recombinant Pichia pastoris containing epoxide hydrolase from Rhodotorula glutinis. Enzym. Microbial Technol. 35, 624-631.
Lee, J.-H. and E. Y. Lee. 2004. Batch production of chiral epichlorohydrin by enantioselective hydrolysis reaction using Rhodosporidium toruloides. Kor. J. Biotechnol. Bioeng. 19, 38-41.
Doderer. K., S. Lutz-Wahl, B. Hauer and R. D. Schmid. 2003. Spectrophotometric assay for epoxide hydrolase activity toward any epoxide. Anal. Biochem. 321, 131-134.
Kasai, N., T. Suzuki, Y. Furukawa. 1998. Chiral C3 epoxides and halohydrins: their preparation and synthetic application. J. Mol. Cat. B: Enz. 4, 237-252.
Lee, E. Y., S.-S. Yoo, H. S. Kim, S. J. Lee, Y.-K. Oh and S. Park. 2004. Production of (S)-styrene oxide by recombinant Pichia pastoris containing epoxide hydrolase from Rhodotorula glutinis. Enzym. Microbial Technol. 35, 624-631.
Lee, J.-H. and E. Y. Lee. 2004. Batch production of chiral epichlorohydrin by enantioselective hydrolysis reaction using Rhodosporidium toruloides. Kor. J. Biotechnol. Bioeng. 19, 38-41.
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UCI(KEPA) : I410-ECN-0101-2009-470-015374984