지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Although valuable microbes have been isolated from the soil for the various productions of useful components, the microbes which can be cultivated in the laboratory are only 0.1~1% of all microbes. To solve this problem, the study has recently been tried for making the valuable components from the environment by directly separating unculturable micrbial DNA in the soil. But it is known that humic acid originated from the soil interrupts various restriction enzymes and molecular biological process. Thus, in order to prevent these problems, this study modified the method separated soil DNA with phenol, CTAB and PEG. In order to compare the degree of purity for each DNA and the molecular biological application process, A_(260)/A_(280) ratio, restriction enzymes, and PCR were performed. In case of DNA by the modified method, total yield of DNA was lower but A_(260)/A_(280) ratio was higher than the previously reported methods. It was confirmed that the degree of purity is improved by the modified method. But it was not cut off by all kinds of tested restriction enzymes because of the operation of a very small amount of interrupting substances. When PCR was operated with each diluted DNA in different concentrations and GAPDH primer, the DNA by the modified method could be processed for PCR in the concentration of 100 times higher than by the previously reported separation method. Therefore, this experiment can find out the possibility of utilization for the unknown substances by effectively removing the harmful materials including humic acid and help establishing metagenomic DNA library from the soil DNA having the high degree of purity.
목차
Abstract
실험재료 및 실험방법
결과
고찰
감사의 글
참고문헌
참고문헌 (15)
참고문헌 신청
Allsop, A., and R. Illingworth. 2002. The impact of genomics and related technologies on the search for new antibiotics. J. Appl. Microbiol. 92(1), 7-12.
Allsop, A. E. 1998. New antibiotic discovery, novel screens, novel targets and impact of microbial genomics. Curr. Opin. Microbiol. 1(5), 530-534.
Brady, S. F., and J. Clardy. 2000. Long-chain N-acyl amino acid antibiotics isolated from heterologously expressed environmental DNA. J. Am. Chem. Soc. 122, 12903-12904.
Brambilla, E., H. Hippe, A. Hagelstein, B. J. Tindall, and E. Stackebrandt. 2001. 16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica. Extremophiles 5, 23-33.
Handelsman, J., M. R. Rondon, S. F. Brady, J. Clardy, and R. M. Goodman. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5, R245-249.
Allsop, A. E. 1998. New antibiotic discovery, novel screens, novel targets and impact of microbial genomics. Curr. Opin. Microbiol. 1(5), 530-534.
Brady, S. F., and J. Clardy. 2000. Long-chain N-acyl amino acid antibiotics isolated from heterologously expressed environmental DNA. J. Am. Chem. Soc. 122, 12903-12904.
Brambilla, E., H. Hippe, A. Hagelstein, B. J. Tindall, and E. Stackebrandt. 2001. 16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica. Extremophiles 5, 23-33.
Handelsman, J., M. R. Rondon, S. F. Brady, J. Clardy, and R. M. Goodman. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5, R245-249.
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Improved DNA Extraction Method for Molecular Diagnosis from Smaller numbers of Cells
대한임상검사과학회지
2014 .01
-
Relationship Between Genome Similarity and DNA-DNA Hybridization Among Closely Related Bacteria
Journal of Microbiology and Biotechnology
2007 .01
-
토양으로부터 DNA의 효과적인 분리
한국생명과학회 심포지움
2004 .10
-
DNA Polymerase 의 구조 및 기능 연구 - DNA 염기서열 결정에 응용 - ( Structural and Functional Aspects of DNA Polymerase - Applications to DNA Sequencing - )
생명과학
1993 .10
-
DNA-based information storage
한국진공학회 학술발표회초록집
2017 .02
-
Evaluation of two DNA extraction methods on exhumed bone samples : Ultrafiltration versus column affinity
분석과학
2008 .08
-
DNA-DNA hybridization에 의한 Bacillus속 균의 동정
한국동물학회 학술대회
1998 .01
-
Extension of a 5'- or 3'-end Genomic DNA Sequence by a Single PCR Amplification
조선자연과학논문집
2008 .01
-
DNA Variation and Disease
한국유전체학회 Conference
2005 .01
-
송이의 Genomic DNA에 특이적인 Probe
한국균학회지
1999 .01
-
Optimization of DNA Extraction from a Single Living Ciliate for Stable and Repetitive PCR Amplification
Animal cells and systems
2009 .01
-
PCR에 의한 DNA 증폭에 미치는 온도와 Cycle 수
대한임상검사과학회지
2004 .01
-
Functional Genomics of Rice by T-DNA Tagging
한국유전체학회 Conference
2002 .01
-
Preparation of PCR-ready Genomic DNA from Saliva
한국동물학회 학술대회
2000 .01
-
유전자 발현 조절과 DNA 3차원적 구조와의 관계
한국식물학회 심포지움
1987 .01
-
Mycobacterium spp.의 신속한 동정을 위한 DNA-DNA Hybridization 조건의 확립
The Journal of the Korean Society for Microbiology
1999 .04
-
말 DNA Typing을 위한 PCR 혼합마커 제작
한국분석과학회 학술대회
2008 .11
-
Expression of DNA-dependent Protein Kinase and Its Relationship with Epidermal Growth Factor Receptor Signaling in Metastatic Cancer Cell Lines
생명과학회지
2005 .06
-
DNA-based data encoding system
한국진공학회 학술발표회초록집
2016 .08
-
T-DNA Integration Patterns in Transgenic Tobacco Plants
식물학회지
2004 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
DNA 이중나선구조에서의 오류 검출 및 복구
김석환
,
허창우
한국정보통신학회논문지
2011
.11
결핵진단을 위한 DNA 영상 자동판별 시스템
김택현
,
최원준
,
남영광
외 1명
한국멀티미디어학회 학술발표논문집
2009
.05
총기 흔적흔에서의 low copy number(LCN) DNA 검출에 관한 연구
전충현
,
박성우
분석과학
2011
.02
유전자재조합 감자의 검정을 위한 DNA분리 및 PCR검출의 최적조건 탐색
신원선
,
김명희
한국식품과학회지
2003
.08
홍조류인 한국산 김종에서의 염색체 DNA 분리방법
최학선
,
최경희
,
이춘환
외 1명
한국식품영양과학회지
1999
.01
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-470-015375810