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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.6
- 수록면
- 127 - 130 (4page)
이용수
초록· 키워드
오류제보하기
It is important to coordinated interaction among neurons, astrocytes and endothelial cells to maintain the function of brain. To study their regulatory mechanisms in vitro system, the co-culture system among the isolated cells from brain may be needed. However, the method for purifying brain microvascular endothelial cells(BMEC) for culture have not established yet. In this study, the proper culture methods of mice cells using two different strains, CD1 and C57BL6, to obtain the pure and plentiful endothelial cells were described. The flatted-round forms of CD1 endothelial cells grew on the collagen-IV coating plates, while the purified cells from C57 mice preferred type collagen-I dishes for their growth. Both cells displayed anti-PECAM-1 (CD31) and von Willebrand Factor immuno-reactivity. These results indicated that different coating materials not only improve attachment of isolated cells but also promoting growth of cells, suggesting that this method of purifying murine Brain microvascular endothelial cells (BMEC) provides a suitable model to investigate blood-brain-barrier (BBB) properties within neurovascular unit in vitro.
목차
영어초록
서론
REFERENCES
참고문헌 (13)
참고문헌 신청
[학술지(정기간행물)] - N,Ichikawa / 1996 / Isolation and primary culture of rat cerebral microvascular endothelial cells for studying drug transport in vitro / J Pharmacol Toxicol Meth. 36 : 45 ~ 52
[학술지(정기간행물)] - RN,Kalaria / 2002 / Small vessel disease and Alzheimer's dementia: pathlogical conditions / Cerebrovasc 13 : 48 ~ 52
[학술지(정기간행물)] - S,Li / 2003 / Culture of murine brain microvascular endothelial cells that maintain expression and cytosckeletal association of tight junction assoxiated proteins / In Vitro Cell Dev Biol. 39 : 313 ~ 320
[학술지(정기간행물)] - Lee, SR / 2004 / Extracellular proteolytic pathophysiology in the neurovascular unit after stroke / Neurol Res. 26 : 854 ~ 861
[학술지(정기간행물)] - Lee, SR, / 2004 / Induction of caspase-mediated cell death by matrix metalloproteinases in cerebral endothelial cells after hypoxia-reoxygenation / J Cerebr Blood Flow Metab 24 : 720 ~ 727
[학술지(정기간행물)] - RN,Kalaria / 2002 / Small vessel disease and Alzheimer's dementia: pathlogical conditions / Cerebrovasc 13 : 48 ~ 52
[학술지(정기간행물)] - S,Li / 2003 / Culture of murine brain microvascular endothelial cells that maintain expression and cytosckeletal association of tight junction assoxiated proteins / In Vitro Cell Dev Biol. 39 : 313 ~ 320
[학술지(정기간행물)] - Lee, SR / 2004 / Extracellular proteolytic pathophysiology in the neurovascular unit after stroke / Neurol Res. 26 : 854 ~ 861
[학술지(정기간행물)] - Lee, SR, / 2004 / Induction of caspase-mediated cell death by matrix metalloproteinases in cerebral endothelial cells after hypoxia-reoxygenation / J Cerebr Blood Flow Metab 24 : 720 ~ 727
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UCI(KEPA) : I410-ECN-0101-2009-510-017357186