지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.45 No.4
- 발행연도
- 2004.8
- 수록면
- 194 - 200 (7page)
이용수
초록· 키워드
오류제보하기
Preliminary primer test for 33 Asian pears (Pyrus spp.) and one outgroup, Prunus persica cv. Hakuto, revealed eighteen primers among sixty Operon random primers. Operon 10-mer primer kit A was useful to analyze pear genome in that nine primers among preliminarily selected 18 primers were in A series. Among the selected primers, 14 primers contained 60% GC composition and produced distinct and reproducible bands at 36 annealing temperature. Most of the Asian pears could be identified with the combination of more than two randomly amplified polymorphic DNA (RAPD) markers and the nine taxa could be distinguished using single RAPD marker. Two more single markers were selected in Fauri pea pear (A01-1390(+) and C07-740(+)), Indian wild pear (A12-2370(+) and E09-1320(?)), and ‘Niitaka’ (A20-950(?), C07-520(+), and E06-1810(?)). Simple matching similarity was calculated and compared between each of the 33 Asian pears and the outgroup, Hakuto peach using 130 RAPD markers. The calculated simple matching similarity coefficient was converted to Neis genetic distance to construct Neighbor- Joining tree to complete the cladogram. The Asian pears were differentiated to four directions from the outgroup. First, three pea pears including Faury, Callery, and Japanese pea pear were divided as ancestor species of genus Pyrus. Asian pea pears including Callery pea pear were assumed to be the descendants of the primitive Pyrus stock. Sand pear and P. hondoensis were divided to the next branch from the root. However, the rest of pear including P. pyrifolia differentiated in the lower progress. The third group including Indian wild pear and Ussurian pea pear branched out. Finally, Ussuri pear and most of the native and cultivated pears were differentiated as a big group followed by P. aromatica, Chinese white pear, and P. phaeocarpa. P. pyrifolia, P. ussuriensis, and P. fauriei were confirmed to be three principal species in Korea.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
Literature Cited
참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] - / 1993 / Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers Application to the mosquito Aedes aegypti
[학술지(정기간행물)] - / 1974 / Rosaceae chemotaxonomy and the origins of the Pomoideas
[학술지(정기간행물)] - / 1973 / Numerical taxonomic studies of genus Pyrus using both chemical and botanical characters
[학술지(정기간행물)] - / 1995a / Classification of native and cultivated Pyrus species in Korea by cluster analysis of morphological characters 36 : 678 ~ 683
[학술지(정기간행물)] - / 1995b / Classification of native and cultivated Pyrus species in Korea by isozyme band patterns 36 : 829 ~ 835
[학술지(정기간행물)] - / 1974 / Rosaceae chemotaxonomy and the origins of the Pomoideas
[학술지(정기간행물)] - / 1973 / Numerical taxonomic studies of genus Pyrus using both chemical and botanical characters
[학술지(정기간행물)] - / 1995a / Classification of native and cultivated Pyrus species in Korea by cluster analysis of morphological characters 36 : 678 ~ 683
[학술지(정기간행물)] - / 1995b / Classification of native and cultivated Pyrus species in Korea by isozyme band patterns 36 : 829 ~ 835
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UCI(KEPA) : I410-ECN-0101-2009-525-016908360