메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색
질문

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.1
발행연도
2005.2
수록면
33 - 38 (6page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색
질문

이 논문의 연구 히스토리 (3)

초록· 키워드

오류제보하기
Double-transformed lettuce plants were obtained from single-transformed lettuce plants with FHV vector (pILTAB 357 containing full length of human H-chain ferritin and nptII genes). Leaf disks from the singletransformed lettuce were transformed serially with FLV vector (pCAMBIA 1302 containing full length of human L-chain ferritin and hpt genes) and selected onto medium containing 50 ㎎ㆍL?¹ kanamycin, 5 ㎎ㆍL?¹ hygromycin, and 200 ㎎ㆍL?¹ cefotaxim. Green shoots were rooted in root-induction medium and regenerated plants were grown in a greenhouse. Polymerase chain reaction (PCR) analysis revealed the presence of the expected 600 and 530 bp products for human H-chain and L-chain ferritin genes, respectively. Reverse transcription-PCR analysis showed that the two types of ferritin genes could be expressed in the double-transformed plants. Most double-transformed lettuce plants except HL10 and HL12 had higher plant height differences than non-transformed plants. HL2, HL8, and HL9 were two times higher Fe concentration than the non-transformed plants, but HL5 and HL23 showed half of Fe concentration than the non-transformed plants. In this study, the two types of human ferritin genes did not have cooperative roles in Fe uptake mechanism in lettuce plants.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited

참고문헌 (21)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

이 논문과 함께 이용한 논문

최근 본 자료

전체보기

댓글(0)

0

UCI(KEPA) : I410-ECN-0101-2009-525-016909130