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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.6
발행연도
2005.12
수록면
351 - 355 (5page)

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Purpose of this study was to develop functional lettuce with higher Fe-storage protein by transfer of rice ferritin gene. The rice ferritin gene was introduced into lettuce (Lactuca sativa L.) plants by Agrobacterium-mediated transformation. The rice ferritin gene was cloned into plant binary vector, pBI101-hm, harboring cauliflower mosaic virus 35S promoter and nopalin synthase terminator. After infection with A. tumefaciens LBA4404 containing pBI101-hm, transformed shoots were induced from tissues on Murashige and Skoog (MS) media supplemented with 0.1 ㎎ㆍℓ?¹ naphthaleneacetic acid, 1.5 ㎎ㆍℓ?¹ benzyladenine, 5 ㎎ㆍℓ?¹ hygromycin, and 200 ㎎ㆍℓ?¹ cefotaxim. Induced shoots were rooted in MS media supplemented with 5 ㎎ㆍℓ?¹ hygromycin and 200 ㎎ㆍℓ?¹ cefotaxim. To confirm the transfer of rice ferritin gene to the genome of lettuce plants, polymerase chain reaction (PCR) analysis was conducted using specific primers of rice ferritin gene. A PCR fragment of 750 bp was obtained from each transformed plants, which was the predicted size of rice ferritin gene but not from non-transformed plants. Reverse transcription-PCR results showed the expression of transgene from transgenic plants. Inductively coupled plasma spectrometry analysis for determination of Fe concentration showed that transgenic lettuce with rice ferritin genes had higher concentration than non-transgenic lettuce.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited

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