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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.47 No.4
발행연도
2006.8
수록면
222 - 229 (8page)

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Dihydroflavonol 4-reductase (DFR) is a key enzyme involved in anthocyanin biosynthesis in aster. DFR catalyses the reduction of dihydroflavonols to leucoanthocyanidins in the anthocyanin biosynthesis pathway. A heterologous cDNA probe from Petunia hybrida was used to isolate DFR-encoding cDNA clone from summer aster (Callistephus chinensis). Comparison of the coding region of this DFR cDNA sequence including the sequences of Zea mays, Anthirrinum majus, P. hybrida, Matthiola incana, Dianthus caryophyllus, and Rosa hybrida reveals a similarity higher than 61 % at the nucleotide level. The DFR transcript is G/C rich in monocotyledonous plants show a strong codon bias preferring codons with a G or C in the third position. The function of this nucleotide sequence was verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by Northern blotting with mRNA from wild type and mutant plants, and by in vitro expression yielding an enzymatically active reductase. Genomic Southern blot analysis showed the presence of one gene for DFR in C. chinensis. Northern blot analysis of the DFR wild-type and mutant lines strongly suggested that the lack of DFR activity in the stable acyanic mutant 10h may be caused by a transcriptional block of the DFR gene.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Literature Cited

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