지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.48 No.5
- 발행연도
- 2007.10
- 수록면
- 265 - 269 (5page)
이용수
초록· 키워드
오류제보하기
Autoexcision strategy was investigated to remove an nptⅡ marker gene from transgenic tobacco. In the autoexcision strategy, the cre gene, under the control of a heat shock-inducible promoter, was excised along with the nptⅡ marker gene. Although no direct relation can be proven between the phenotype and cre expression in some plants, expressing the cre gene driven by a Ca㎹ 35S promoter displayed growth retardation and a distinct pattern of chlorosis in the leaves. The recombinant event could be detected because it places the Ca㎹ 35S promoter of the nptⅡ gene adjacent to a promoterless gusA gene. As a result the gusA gene was activated and its expression could be visualized. In the autoexcision strategy, 20 lines showed low or even could not detect GUS activity before heat treatment. However, after heat treatment, the degree of GUS expression increased in 16 lines after heat treatment. In this case, recombination occurred as high efficiency. Our results show that a transient heat shock treatment is sufficient for inducing cre and excising the cre and nptⅡ gene. Molecular analysis and GUS assay confirmed that marker gene removal was precise, complete and stable.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Literature cited
참고문헌 (15)
참고문헌 신청
1. [학술지(정기간행물)] - Bayley / / and D.W. Ow. 1992. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox sitespecific recombination system. Plant Mol. Biol. 18 : 353 ~ 361
2. [학술지(정기간행물)] - Dale / / E.C. and D.W. Ow. 1991. Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88 : 10558 ~ 10562
3. [학술지(정기간행물)] - Hoff, T / 2001 / recombinase- mediated transcriptional induction system in transgenic plants. 45 : 41 ~ 49
4. [학술지(정기간행물)] - Jefferson / / R.A. 1987. Assaying chimeric genes in plants : 387 ~ 405
5. [학술지(정기간행물)] - Medberry, S.L / 1995 / Intra- chromosomal rearrangements generated by Cre-loxsite-specific recombination. 23 : 485 ~ 490
2. [학술지(정기간행물)] - Dale / / E.C. and D.W. Ow. 1991. Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88 : 10558 ~ 10562
3. [학술지(정기간행물)] - Hoff, T / 2001 / recombinase- mediated transcriptional induction system in transgenic plants. 45 : 41 ~ 49
4. [학술지(정기간행물)] - Jefferson / / R.A. 1987. Assaying chimeric genes in plants : 387 ~ 405
5. [학술지(정기간행물)] - Medberry, S.L / 1995 / Intra- chromosomal rearrangements generated by Cre-loxsite-specific recombination. 23 : 485 ~ 490
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