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The effect of the fruiting bodies of Paecilomyces japonica (PJ), grown on silk-worm larvae on the changes of ultraviolet (UV) light B radiation-induced apoptotic sunburn cell (SBC) and epidermal ATPase-positive dendritic cell (DC) in SKH1-hr or ICR mouse were investigated. The mice were treated with UVB (200 mJ/㎠) and were sacrificed 24 hours later. PJ (50 ㎎/㎏ of body weight) or vehicle (saline) was given i.p. at 36 and 12 hours before irradiation, and 30 minutes after irradiation or PJ cream (0.2%) or cream base (vehicle) was topically treated at 24 hours and 15 minutes before irradiation, and immediately after irradiation. The skin of SKH1-hr mouse prepared from the back of untreated mice exhibited about 0.3 SBC/㎝ length of epidermis, and 24 hours after UV irradiation, the applied areas show an increased number of SBCs. But the frequency of UVB-induced SBC formation was reduced by intraperitoneal injection of PJ extract (p < 0.01). The numbers of DC in normal ICR mouse were 628.00± 51.56 or 663.20± 62.58 per ㎟ of ear epidermis. By 1 day after UVB treatment, the number of ATPase-positive cells/mrn? were decreased by 39.0% or 27.1% in i.p. or topical application group with vehicle. Treatment of PJ was associated with reductions of 30.8% in i.p, group (p < 0.05) and 8.4% in topical application group in the number of ATPase positive cells compared with the irradiation control group. The results presented herein that PJ administration could reduce the extent of skin damages produced by UVB.

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UCI(KEPA) : I410-ECN-0101-2009-510-016363022