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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.9
- 수록면
- 321 - 326 (6page)
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To investigate the effect of fetal cardiac cells on the differentiation of embryonic stem cells (ESCs) into cardiomyocytes, Mouse ESCs were co-cultured with heart cells prepared from fetus aged 11.5, 12.5, 13.5 or 14.5 days postcoitum (dpc). The fetal mouse heart was from ICR and 129 strains from which KH2 ES cell line was derived. In the present study, KH2 cells expressing EGFP directed by α-myosin heavy chain gene promoter were co-cultured with fetal heart isolated at different fetal stages. The cardiomyocytes derived from ESC in the co-culture could be identified by their EGFP expression. The EGFP expressing cells which were confirmed to be cardiomyocytes were measured as an indicator of cardiac cell differentiation of ESC. The fetal cardiac cells from 12.5 dpc were more effective in cardiac cell differentiation of ESC than the cells from fetus aged 11.5, 13.5 or 14.5 dpc. Fetal heart from 129 strain which is immunologically compatible with KH2 cells showed more strong cardiac cell differentiation induction activity compared with that from ICR mice. As the results, we demonstrated that fetal hearts at different developmental stages have different cardiac cell differentiation induction activity from ESC implying that fetal heart expresses cardiac cell differentiation induction factor(s).
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참고문헌 (24)
참고문헌 신청
Baharvand, H , 2006 , Ultrastructural comparison of developing mouse embryonic stem cell- and in vivo derived cardiomyocytes 30 : 800 ~ 807
Bai, X , 2007 , Genetically selected stem cells from human adipose tissue express cardiac markers 353 : 665 ~ 671
Czyz, J , 2003 , Potential of embryonic and adult stem cells in vitro 384 : 1391 ~ 409
Davani, S , 2005 , Can stem cells mend a broken heart? 65 : 305 ~ 316
Deb, A , 2003 , Bone marrow-derived cardiomyocytes are present in the adult human heart: A study of gender-mismatched bone marrow transplantation recipients 107 : 1247 ~ 1249
Bai, X , 2007 , Genetically selected stem cells from human adipose tissue express cardiac markers 353 : 665 ~ 671
Czyz, J , 2003 , Potential of embryonic and adult stem cells in vitro 384 : 1391 ~ 409
Davani, S , 2005 , Can stem cells mend a broken heart? 65 : 305 ~ 316
Deb, A , 2003 , Bone marrow-derived cardiomyocytes are present in the adult human heart: A study of gender-mismatched bone marrow transplantation recipients 107 : 1247 ~ 1249
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UCI(KEPA) : I410-ECN-0101-2009-510-016364307