지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.49 No.1
- 발행연도
- 2008.2
- 수록면
- 52 - 57 (6page)
이용수
초록· 키워드
오류제보하기
In vitro plantlets of potato genotype ‘F9-99’ infected with potato leaf roll virus (PLRV) and potato virus Y (PVY) were used for virus elimination studies using electric current and chemical treatments. Virus status of in vitro plantlets was determined by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR) before and after treatment. The highest (62.5%) and the lowest (21.9%) regeneration rates were observed from the shoot buds excised after electric treatments with 10 miliampers (mA) for 5 min and 15 mA for 10 min, respectively; also, there were no significant effect on plant growth after electric treatment, except for very high mA for long time. As for virus elimination, current supplied at 10 mA for 5 min resulted in the highest rate of PLRV (46.7%) and PVY (40%) elimination, whereas the lowest virus elimination rate was from the lowest electric current applied for short duration in both of the potato viruses. Study also showed that the plantlets regenerated after electric treatments had higher percentages of virus-free from the shoot buds excision than the nodal cuttings. In the case of electric and chemical treatment combination, the highest rates of PLRV (67.2%) and PVY (62.8%) free plantlets were obtained from shoot buds excised after electrotherapy and cultured on MS medium containing 3% sucrose, 0.7% agar and addition of ribavirin (20 ㎎ㆍℓ?¹) and acetylsalicylic acid (ASA) (10?? M). However, the virus concentration was highly reduced by the addition of ribavirin in the culture medium and the regeneration rates were the highest (72.7%) from the ASA added treatment. Leaf and tuber morphology of plant regenerated from electrotherapy and chemotherapy were similar to those from non-treated control plants. Similar results of virus elimination were obtained under in vitro and in vivo conditions after treatment. Therefore, this study confirmed that electrotherapy (10 mA for 5 min) with the addition of ribavirin (20 ㎎ㆍℓ?¹) and ASA (10?? M) into the cultural medium was the most effective for virus elimination from the virus infected plantlets under in vitro condition and acetylsalicylic acid (ASA).
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgement
Literature Cited
참고문헌 (25)
참고문헌 신청
1. [단행본] - Beemster, A.B.R / 1972 / Potato viruses; properties and symptoms In: Viruses of potatoes and seed potato production / Wangeningen : 115 ~ 143
2. [학술지(정기간행물)] - Campbell, R.N / 1980 / Effects of benomyl and ribavirin on the lettuce big vein agent and its transmission.Phytopathol.70:1190-1192 / Phytopathol 70 : 1190 ~ 1192
3. [학술지(정기간행물)] - Cassels, A.C. / 1982 / The elimination of potato viruses X, Y, S, and M in meristem and explants culture of potato in the presence of virazole / Potato Res 25 : 165 ~ 173
4. [학술지(정기간행물)] - Clark, M.F. / 1977 / Characteristics of the micro plate method of enzyme linked immunosorbent assay for the detection of potato viruses / J. Gen. Virol 34 : 475 ~ 483
5. [학술지(정기간행물)] - Yi Lan Fang / 2005 / Utilization of Single Nodal Cuttings and Therapies for Eradicating Double-Infected Potato Virus (PLRV, PVY) from In Vitro Plantlets of Potato (Solanum tuberosum) / Horticulture Environment and Biotechnology 46 (2) : 119 ~ 125
2. [학술지(정기간행물)] - Campbell, R.N / 1980 / Effects of benomyl and ribavirin on the lettuce big vein agent and its transmission.Phytopathol.70:1190-1192 / Phytopathol 70 : 1190 ~ 1192
3. [학술지(정기간행물)] - Cassels, A.C. / 1982 / The elimination of potato viruses X, Y, S, and M in meristem and explants culture of potato in the presence of virazole / Potato Res 25 : 165 ~ 173
4. [학술지(정기간행물)] - Clark, M.F. / 1977 / Characteristics of the micro plate method of enzyme linked immunosorbent assay for the detection of potato viruses / J. Gen. Virol 34 : 475 ~ 483
5. [학술지(정기간행물)] - Yi Lan Fang / 2005 / Utilization of Single Nodal Cuttings and Therapies for Eradicating Double-Infected Potato Virus (PLRV, PVY) from In Vitro Plantlets of Potato (Solanum tuberosum) / Horticulture Environment and Biotechnology 46 (2) : 119 ~ 125
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UCI(KEPA) : I410-ECN-0101-2009-525-016412896