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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.5
발행연도
2009.10
수록면
467 - 471 (5page)

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A method was developed for rapid micropropagation of Valeriana officinalis through shoot regeneration from calli derived from leaf and petiole explants. The petiole and leaf segments were cultured on Murashige and Skoog (MS) medium supplemented with different combinations of different auxins [2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphtaleneacetic acid (NAA), picloram (Pic)], and Kinetin (Kin) for callus induction. Callus production was the highest at 1.0 ㎎ㆍL?¹ 2,4-D being 100 and 98% from leaf and petiole explants, respectively. The produced calli were transferred to a medium supplemented with various concentrations of BA (0.1, 0.2, and 0.4 ㎎ㆍL?¹) or Kin (0.1, 0.2, and 0.4, ㎎ㆍL?¹) for shoot regeneration. The addition of 0.2 ㎎ㆍL?¹ BA or Kin increased the frequency of shoot regeneration in both petiole and leaf callus segments. The highest number of adventitious shoots (13 per callus segments) formed in leaf derived callus. The optimal rooting was observed on MS medium supplemented with 1 ㎎ㆍL?¹ NAA, on which 100% of the regenerated shoots developed roots with an average of 10.7 roots per shoot within 3 weeks. The in vitro raised plantlets were acclimatized and transferred to greenhouse with 80% success. This in vitro propagation protocol could be useful for conservation as well as mass propagation of this medicinal plant.

목차

Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgement
Literature Cited

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UCI(KEPA) : I410-ECN-0101-2009-525-018979930