The present study was made to evaluate in vitro antibacterial and antioxidant activities of Bokbunja (Rubus coreanus Miquel), Bakha(Mentha arvensis var. piperascens), and African marigold (Tagetes erects) extracts and the effect of their dietary supplementation on the productivity and physiological response in poultry. Exp. 1 was carried out to investigate in vitro antimicrobial and antioxidant activities of Bokbunja, Bakha, and African marigold extracts. In vitro antimicrobial activity of Bokbunja, Bakha, and African marigold extracts were examined against five kinds of pathogenic bacteria. There were some potential antimicrobial activities against Salmonella typhitnurium, Enterococcus faecalis, Escherichia coli, Salmonella gallinarum. Listeria monocytogenes in all of the extracts. All of the plant extracts had antioxidant activity in vitro examination. In DPPH radical scavenging assay, it was shown all of Bokbunja, Bakha, and African marigold extracts had antioxidant properties, although their inhibitory potencies were not so strong as those of control (BHA and a-tocopherol). African marigold extract had the highest DPPH radical scavenging activity among the three extracts. The results indicate that Bokbunja, Bakha, and African marigold extracts might have functions as biopreservative, antioxidant agent and the alternatives for antibiotics in poultry feed. Exp. 2 was undertaken to evaluate the dietary effects of Bokbunja, Bakha, and African marigold extracts on laying performance, egg quality, prevention of egg oxidation in storage. A total of one-hundred eighty nine 42-wk-old Hy-Line Brown layers were divided into seven groups and fed the control diet or each experimental diet (containing 0.3 or 0.5 % Bokbunja, 0.3 or 0.5 % Bakha, and 0.3 or 0.5 % African marigold extracts) for 6 weeks. The egg weights of the birds fed the diet containing 0.3 % African marigold extracts were significantly increased compared to that of control(P<0.05). There was no significant difference in egg production. Egg shell color and Haugh unit were improved by the dietary treatments significantly(P<0.05), and egg yolk color was significantly increased by supplements of Bakha or African marigold extract(P<0.05). The numbers of cecal Coli forms in groups fed the diets containing the three plant extracts were tended to be decreased. The MDA contents in stored eggs were significantly lowered by feeding the three plant extracts(P<0.05). The Haugh units of the treatment groups fed all of the extracts was tended to be increased after 7 days of storage and significantly increased after 14 days of storage compared to that of control. These results indicated that dietary Korean traditional medicinal plants (Bokbunja, Bakha, and African marigold extracts) improved egg quality such as egg color, egg shell color, and Haugh unit, implying that Bokbunja, Bakha. and African marigold extracts may decrease lipid oxidation in eggs, and improve profiles of cecal microflora. In conclusion they can be used as potential alternative substances to replace antibiotics and antioxidant agent for poultry feeding. Exp. 3 was conducted to evaluate dietary effects of African marigold extract on laying performance, egg quality, prevention of egg oxidation in storage and lutein transfer from dietary African marigold into egg yolk. A total of one-hundred eighty nine 42-wk-old Hy-Line Brown layers were divided into seven groups and fed control diet or each experimental diet containing 0.1, 0.3, 0.5, 1.0, 1.5, 3.0 % African marigold extract for 6 weeks. The egg production of the birds fed African marigold extract were increased when compared to that of control, although there was no significant difference. Egg quality including egg shell, egg yolk color and Haugh unit were significantly improved by the dietary treatments of African marigold extract(P<0.05). The MDA contents in stored egg was lowered by feeding the African marigold extract significantly (P<0.05). The Haugh units in the groups fed all the three extracts were tended to be increased after 7 days of storage and significantly increased after 14 days of storage(P<0.05). The concentration of lutein in egg york was linearly increased by adding African marigold extract in layer diet (P<0.05). When 2 % African marigold extract was supplemented to the layer diet, lutein content of egg was increased as much as 1.71 ㎎/60g These results indicated that dietary African marigold extract improved egg quality such as egg color, egg shell color, and Haugh unit. The supplementation increased lutein concentration of egg yolk, and inhibited lipid oxidation in eggs. From these experiments, African marigold extract could be an alternative substances to replace antibiotics and used as natural antioxidant and natural pigment agents for poultry feeding.