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논문 기본 정보

자료유형
학술저널
저자정보
Hyun Gi Kong (동아대학교) Jin-Cheol Kim (한국화학연구원) Gyoung Ja Choi (한국화학연구원) Kwang Youll Lee (동아대학교) Hyun Ju Kim (국립식물검역원) Eul Chul Hwang (동아대학교) Byung Ju Moon (동아대학교) Seon-Woo Lee (동아대학교)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.26 No.2
발행연도
2010.6
수록면
170 - 177 (8page)

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초록· 키워드

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Bacillus licheniformis N1, previously developed as a biofungicide formulation N1E to control gray mold disease of plants, was investigated to study the bacterial traits that may be involved in its biological control activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original formulation N1E. Fractionation of antifungal compounds from the bacterial culture supernatant of B. licheniformis N1 indicated that two different cyclic lipopeptides were responsible for the antimicrobial activity of the N1 strain. These two purified compounds were identified as iturin A and surfactin by HPLC and LCMS. The purified lipopeptides were evaluated for plant disease control activity against seven plant diseases. Crude extracts and purified compounds applied at 500 ㎍/㎖ concentration controlled tomato gray mold, tomato late blight and pepper anthracnose effectively with over 70% disease control value. While iturin showed broad spectrum activity against all tested plant diseases, the control activity by surfactin was limited to tomato gray mold, tomato late blight, and pepper anthracnose. Although antifungal compounds from B. licheniformis N1 exhibited disease control activity, our results suggested that bacterial cells present in the N1E formulation also contribute to the disease control activity together with the antifungal compounds.

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Materials and Methods
Results
Discussion
Acknowledgement
References

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UCI(KEPA) : I410-ECN-0101-2010-481-002965773