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논문 기본 정보

자료유형
학술저널
저자정보
Cai-Yi Wen (Fujian Agriculture and Forestry University) Zhi-Gang Yin (Henan Agricultural University) Kai-Xuan Wang (Henan Agricultural University) Jian-Guang Chen (Henan Agricultural University) Shun-Shan Shen (Henan Agricultural University)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.27 No.4
발행연도
2011.12
수록면
342 - 348 (7page)

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초록· 키워드

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Bacillus subtilis EBS05, an endophytic bacteria strain isolated from a medicinal plant Cinnamomum camphor, can produce antagonistic compounds that effectively inhibit plant pathogenic fungi. The greenhouse experiments showed that wheat sharp eyespot disease (WSED) was reduced by 91.2%, 88.2% and 43.0% after the treatment with fermentation broth, bacteria-free filter and a fungicide fludioxonil, respectively. The culture broth of strain EBS05 can more effectively control WSED than can fludioxonil. The fermentation broth and bacteria-free filter ability to suppress WSED was not significantly different, suggesting that an active secreted substance played a major role in controlling WSED. Separation and purification of the active compounds was carried out by serial processes, including hydrochloric acid (pH 2.0) treatment, methanol extraction and Sephadex LH-20 column chromatography, silica gel column chromatography and reverse-phase high-pressure liquid chromatography (HPLC), respectively. The purified compounds, one of active peaks in the HPLC spectrum, were obtained from the collection. Analysis of the chemical structures by time-of-flight mass spectrometry (TOF-MS) and electrospray ionization mass spectrometry/mass spectrometry (ESI-MS/MS) showed that the active substances produced by the endophytic bacteria EBS05 are mixture of the β-hydroxy-C12~15-Leu? surfactin A isomers with 1035.65 Da, 1021.64 Da, 1007.63 Da and 993.65 Da molecular weights, respectively.

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Materials and Methods
Results
Discussion
Acknowledgement
References

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UCI(KEPA) : I410-ECN-0101-2013-481-001446080