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논문 기본 정보

자료유형
학술저널
저자정보
정행진 (충남대학교) 유성란 (충남대학교) 이준헌 (충남대학교) 도창희 (충남대학교) 서국현 (전남대학교) 류승희 (충청남도축산기술연구소) 상병찬 (충남대학교)
저널정보
충남대학교 농업과학연구소 Korean Journal of Agricultural Science 農業科學硏究 第38卷 第1號
발행연도
2011.3
수록면
45 - 50 (6page)

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초록· 키워드

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This study was conducted to investigate the farm situation about bovine leukemia virus(BLV) infection that greatly influence productivity in dairy cattle and compare the accuracy of diagnosis for BLV infection between PCR and ELISA techniques. Blood samples of 193 heads from 5 herds in Chungnam and Chungbuk area were used to analyze BLV gene and serum, and the results were obtained as follows. The amplified BLV gene in dairy cattle by PCR technique resulted in 226 bp, 596 bp and 434 bp, respectively, for gag, pol and env, which were well amplified. The infection rates of BLV virus diagnosed by PCR and ELISA techniques ranged from 80.55 to 100% and from 22.22 to 86.95%, respectively, and the infection rates among 5 herds were significantly different in both methods (P<0.05). Further, the average infection rates of 5 herds were 87.05 and 63.21%, respectively, for PCR and ELISA techniques. Kappa statistics for examining consistency of diagnosis by PCR and ELISA techniques showed 0.246, which represents low consistency. Consequently, PCR based BLV technique was considered as a corrective measure for diagnosis of BLV infection in Holstein dairy cattle.

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Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
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UCI(KEPA) : I410-ECN-0101-2012-480-004474178