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논문 기본 정보

자료유형
학술저널
저자정보
Won-Bo Shim (Graduate School of Gyeongsang National University) Jung-Sook Kim (Graduate School of Gyeongsang National University) Ji-Young Kim (Graduate School of Gyeongsang National University) Jin-Gil Choi (Graduate School of Gyeongsang National University) Jung-Hyun Je (Graduate School of Gyeongsang National University) Nina Sergeevna Kuzmina (Mechnikov Research Institute of Vaccine and Sera of Russian Academy of Medical Sciences) Sergei Alexandrovich Eremin (M. V. Lomonosov Moscow State University) Duck-Hwa Chung (Graduate School of Gyeongsang National University)
저널정보
한국식품과학회 Food Science and Biotechnology Food Science and Biotechnology vol 17. no 3
발행연도
2008.6
수록면
623 - 630 (8page)

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A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG striptest and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

목차

Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References

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