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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.2
- 수록면
- 15 - 19 (5page)
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This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-Stransferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgments
References
참고문헌 (20)
참고문헌 신청
Mi-Hwa Chun , 2006 , Reduction of Pesticide Residues in the Production of Red Pepper Powder , Food Science and Biotechnology 15 (1) : 57 ~ 62
Se-Mi Kang , 2005 , Fate of Some Pesticides during Brining and Cooking of Chinese Cabbage and Spinach , Food Science and Biotechnology 14 (1) : 77 ~ 81
Abad JM , 1998 , Determination of organophosphorus and carbamate pesticides using a piezoelectric biosensor , Anal. Chem 70 : 2848 ~ 2855
Chang-Hwan Oh , 2006 , Applicability of Using GC-PDD (Pulsed Discharge Detector) for Multiresidual Pesticides Analysis , Food Science and Biotechnology 15 (6) : 959 ~ 966
Song SJ , 2003 , Enzyme immunoassay for on-line sensing of the insecticide imidaclopird residues , J. Korean Soc. Agric. Mach 28 : 505 ~ 510
Se-Mi Kang , 2005 , Fate of Some Pesticides during Brining and Cooking of Chinese Cabbage and Spinach , Food Science and Biotechnology 14 (1) : 77 ~ 81
Abad JM , 1998 , Determination of organophosphorus and carbamate pesticides using a piezoelectric biosensor , Anal. Chem 70 : 2848 ~ 2855
Chang-Hwan Oh , 2006 , Applicability of Using GC-PDD (Pulsed Discharge Detector) for Multiresidual Pesticides Analysis , Food Science and Biotechnology 15 (6) : 959 ~ 966
Song SJ , 2003 , Enzyme immunoassay for on-line sensing of the insecticide imidaclopird residues , J. Korean Soc. Agric. Mach 28 : 505 ~ 510
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UCI(KEPA) : I410-ECN-0101-2013-573-001009875